Neurotrophic factor-secreting cell grafting for cerebral ischemia

Preliminary report

K. Fujiwara, Isao Date, T. Shingo, H. Yoshida, K. Kobayashi, A. Takeuchi, T. Tamiya, T. Ohmoto

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In this experiment, we examined a possible protective effect of encapsulated neurotrophic factor-secreting cell grafting for ischemic injury. We established a basic fibroblast growth factor (bFGF)-secreting cell line by genetic manipulation. We enveloped these cells into polymer capsules, which consist of a semipermeable membrane, and implanted them into the right striatum of rats. At 6 days after implantation, these rats received right middle cerebral artery occlusion (MCAO) using interluminal suture technique. At 24 h after MCAO, rats were sacrificed and their cerebral infarction volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining and image analysis. We found approximately 30% reduction in infarct volume in the encapsulated bFGF-secreting cell grafting groups vs. the encapsulated naive BHK cell grafting group or the without implantation group. We measured bFGF secretion from encapsulated bFGF-secreting cells using enzyme-linked immunosorbent assay (ELISA). The retrieved capsules continued to secrete bFGF. There was no significant difference of bFGF secretion between the capsules before and after transplantation. A large number of viable BHK-bFGF cells was observed within the full length of the retrieved capsule. These results indicate that encapsulated bFGF-secreting cell grafting exerts a protective effect on ischemic injury.

Original languageEnglish
Pages (from-to)419-422
Number of pages4
JournalCell Transplantation
Volume10
Issue number4-5
Publication statusPublished - 2001

Fingerprint

Nerve Growth Factors
Fibroblast Growth Factor 2
Fibroblasts
Brain Ischemia
Capsules
Rats
Middle Cerebral Artery Infarction
Suture Techniques
Intercellular Signaling Peptides and Proteins
Cerebral Infarction
Wounds and Injuries
Ion implantation
Image analysis
Assays
Polymers
Enzymes
Transplantation
Enzyme-Linked Immunosorbent Assay
Cells
Staining and Labeling

Keywords

  • Basic fibroblast growth factor (bFGF)
  • Cell grafting
  • Encapsulation
  • Ischemia

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation

Cite this

Fujiwara, K., Date, I., Shingo, T., Yoshida, H., Kobayashi, K., Takeuchi, A., ... Ohmoto, T. (2001). Neurotrophic factor-secreting cell grafting for cerebral ischemia: Preliminary report. Cell Transplantation, 10(4-5), 419-422.

Neurotrophic factor-secreting cell grafting for cerebral ischemia : Preliminary report. / Fujiwara, K.; Date, Isao; Shingo, T.; Yoshida, H.; Kobayashi, K.; Takeuchi, A.; Tamiya, T.; Ohmoto, T.

In: Cell Transplantation, Vol. 10, No. 4-5, 2001, p. 419-422.

Research output: Contribution to journalArticle

Fujiwara, K, Date, I, Shingo, T, Yoshida, H, Kobayashi, K, Takeuchi, A, Tamiya, T & Ohmoto, T 2001, 'Neurotrophic factor-secreting cell grafting for cerebral ischemia: Preliminary report', Cell Transplantation, vol. 10, no. 4-5, pp. 419-422.
Fujiwara K, Date I, Shingo T, Yoshida H, Kobayashi K, Takeuchi A et al. Neurotrophic factor-secreting cell grafting for cerebral ischemia: Preliminary report. Cell Transplantation. 2001;10(4-5):419-422.
Fujiwara, K. ; Date, Isao ; Shingo, T. ; Yoshida, H. ; Kobayashi, K. ; Takeuchi, A. ; Tamiya, T. ; Ohmoto, T. / Neurotrophic factor-secreting cell grafting for cerebral ischemia : Preliminary report. In: Cell Transplantation. 2001 ; Vol. 10, No. 4-5. pp. 419-422.
@article{386ceaffa2d547c98c6c9052ee6d5261,
title = "Neurotrophic factor-secreting cell grafting for cerebral ischemia: Preliminary report",
abstract = "In this experiment, we examined a possible protective effect of encapsulated neurotrophic factor-secreting cell grafting for ischemic injury. We established a basic fibroblast growth factor (bFGF)-secreting cell line by genetic manipulation. We enveloped these cells into polymer capsules, which consist of a semipermeable membrane, and implanted them into the right striatum of rats. At 6 days after implantation, these rats received right middle cerebral artery occlusion (MCAO) using interluminal suture technique. At 24 h after MCAO, rats were sacrificed and their cerebral infarction volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining and image analysis. We found approximately 30{\%} reduction in infarct volume in the encapsulated bFGF-secreting cell grafting groups vs. the encapsulated naive BHK cell grafting group or the without implantation group. We measured bFGF secretion from encapsulated bFGF-secreting cells using enzyme-linked immunosorbent assay (ELISA). The retrieved capsules continued to secrete bFGF. There was no significant difference of bFGF secretion between the capsules before and after transplantation. A large number of viable BHK-bFGF cells was observed within the full length of the retrieved capsule. These results indicate that encapsulated bFGF-secreting cell grafting exerts a protective effect on ischemic injury.",
keywords = "Basic fibroblast growth factor (bFGF), Cell grafting, Encapsulation, Ischemia",
author = "K. Fujiwara and Isao Date and T. Shingo and H. Yoshida and K. Kobayashi and A. Takeuchi and T. Tamiya and T. Ohmoto",
year = "2001",
language = "English",
volume = "10",
pages = "419--422",
journal = "Cell Transplantation",
issn = "0963-6897",
publisher = "Cognizant Communication Corporation",
number = "4-5",

}

TY - JOUR

T1 - Neurotrophic factor-secreting cell grafting for cerebral ischemia

T2 - Preliminary report

AU - Fujiwara, K.

AU - Date, Isao

AU - Shingo, T.

AU - Yoshida, H.

AU - Kobayashi, K.

AU - Takeuchi, A.

AU - Tamiya, T.

AU - Ohmoto, T.

PY - 2001

Y1 - 2001

N2 - In this experiment, we examined a possible protective effect of encapsulated neurotrophic factor-secreting cell grafting for ischemic injury. We established a basic fibroblast growth factor (bFGF)-secreting cell line by genetic manipulation. We enveloped these cells into polymer capsules, which consist of a semipermeable membrane, and implanted them into the right striatum of rats. At 6 days after implantation, these rats received right middle cerebral artery occlusion (MCAO) using interluminal suture technique. At 24 h after MCAO, rats were sacrificed and their cerebral infarction volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining and image analysis. We found approximately 30% reduction in infarct volume in the encapsulated bFGF-secreting cell grafting groups vs. the encapsulated naive BHK cell grafting group or the without implantation group. We measured bFGF secretion from encapsulated bFGF-secreting cells using enzyme-linked immunosorbent assay (ELISA). The retrieved capsules continued to secrete bFGF. There was no significant difference of bFGF secretion between the capsules before and after transplantation. A large number of viable BHK-bFGF cells was observed within the full length of the retrieved capsule. These results indicate that encapsulated bFGF-secreting cell grafting exerts a protective effect on ischemic injury.

AB - In this experiment, we examined a possible protective effect of encapsulated neurotrophic factor-secreting cell grafting for ischemic injury. We established a basic fibroblast growth factor (bFGF)-secreting cell line by genetic manipulation. We enveloped these cells into polymer capsules, which consist of a semipermeable membrane, and implanted them into the right striatum of rats. At 6 days after implantation, these rats received right middle cerebral artery occlusion (MCAO) using interluminal suture technique. At 24 h after MCAO, rats were sacrificed and their cerebral infarction volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining and image analysis. We found approximately 30% reduction in infarct volume in the encapsulated bFGF-secreting cell grafting groups vs. the encapsulated naive BHK cell grafting group or the without implantation group. We measured bFGF secretion from encapsulated bFGF-secreting cells using enzyme-linked immunosorbent assay (ELISA). The retrieved capsules continued to secrete bFGF. There was no significant difference of bFGF secretion between the capsules before and after transplantation. A large number of viable BHK-bFGF cells was observed within the full length of the retrieved capsule. These results indicate that encapsulated bFGF-secreting cell grafting exerts a protective effect on ischemic injury.

KW - Basic fibroblast growth factor (bFGF)

KW - Cell grafting

KW - Encapsulation

KW - Ischemia

UR - http://www.scopus.com/inward/record.url?scp=0034870493&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034870493&partnerID=8YFLogxK

M3 - Article

VL - 10

SP - 419

EP - 422

JO - Cell Transplantation

JF - Cell Transplantation

SN - 0963-6897

IS - 4-5

ER -