Neurotrophic factor-secreting cell grafting for cerebral ischemia: Preliminary report

K. Fujiwara, I. Date, T. Shingo, H. Yoshida, K. Kobayashi, A. Takeuchi, T. Tamiya, T. Ohmoto

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7 Citations (Scopus)

Abstract

In this experiment, we examined a possible protective effect of encapsulated neurotrophic factor-secreting cell grafting for ischemic injury. We established a basic fibroblast growth factor (bFGF)-secreting cell line by genetic manipulation. We enveloped these cells into polymer capsules, which consist of a semipermeable membrane, and implanted them into the right striatum of rats. At 6 days after implantation, these rats received right middle cerebral artery occlusion (MCAO) using interluminal suture technique. At 24 h after MCAO, rats were sacrificed and their cerebral infarction volume was determined by 2,3,5-triphenyltetrazolium chloride (TTC) staining and image analysis. We found approximately 30% reduction in infarct volume in the encapsulated bFGF-secreting cell grafting groups vs. the encapsulated naive BHK cell grafting group or the without implantation group. We measured bFGF secretion from encapsulated bFGF-secreting cells using enzyme-linked immunosorbent assay (ELISA). The retrieved capsules continued to secrete bFGF. There was no significant difference of bFGF secretion between the capsules before and after transplantation. A large number of viable BHK-bFGF cells was observed within the full length of the retrieved capsule. These results indicate that encapsulated bFGF-secreting cell grafting exerts a protective effect on ischemic injury.

Original languageEnglish
Pages (from-to)419-422
Number of pages4
JournalCell Transplantation
Volume10
Issue number4-5
DOIs
Publication statusPublished - 2001

Keywords

  • Basic fibroblast growth factor (bFGF)
  • Cell grafting
  • Encapsulation
  • Ischemia

ASJC Scopus subject areas

  • Biomedical Engineering
  • Cell Biology
  • Transplantation

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  • Cite this

    Fujiwara, K., Date, I., Shingo, T., Yoshida, H., Kobayashi, K., Takeuchi, A., Tamiya, T., & Ohmoto, T. (2001). Neurotrophic factor-secreting cell grafting for cerebral ischemia: Preliminary report. Cell Transplantation, 10(4-5), 419-422. https://doi.org/10.3727/000000001783986666