Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury

R. Terayama, Y. Bando, K. Murakami, K. Kato, M. Kishibe, S. Yoshida

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Previous studies indicated that the expression of neuropsin, a serine protease, is induced in mature oligodendrocytes after injury to the CNS. The pathophysiology of spinal cord injury (SCI) involves primary and secondary mechanisms, the latter contributing further to permanent losses of function. To explore the role of neuropsin after SCI, histochemical and behavioral analyses were performed in wild-type (WT) and neuropsin-deficient (neuropsin-/-) mice using a crush injury model, a well-characterized and consistently reproducible model of SCI. In situ hybridization revealed that neuropsin mRNA expression was induced in the spinal cord white matter from WT mice after crush SCI, peaking at day 4. Neuropsin-/- mice showed attenuated demyelination, oligodendrocyte death, and axonal damage after SCI. Although axonal degeneration in the corticospinal tract was obvious caudal to the lesion site in both strains of mice after SCI, the number of surviving nerve fibers caudal to the lesion was significantly larger in neuropsin-/- mice than WT mice. Behavioral analysis revealed that the recovery at days 10-42 was significantly improved in neuropsin-/- mice compared with WT mice in spite of the severe initial hindlimb impairments due to SCI in both strains. These observations suggest that neuropsin is involved in the secondary phase of the pathogenesis of SCI mediated by demyelination, oligodendrocyte death, and axonal degeneration.

Original languageEnglish
Pages (from-to)175-187
Number of pages13
JournalNeuroscience
Volume148
Issue number1
DOIs
Publication statusPublished - Aug 10 2007

Fingerprint

Oligodendroglia
Demyelinating Diseases
Spinal Cord Injuries
Pyramidal Tracts
Serine Proteases
Hindlimb
Nerve Fibers
In Situ Hybridization
Spinal Cord
Messenger RNA
Mouse Prss19 protein
Wounds and Injuries

Keywords

  • behavior
  • immunofluorescence labeling
  • in situ hybridization
  • secondary injury
  • serine protease
  • tract tracing

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury. / Terayama, R.; Bando, Y.; Murakami, K.; Kato, K.; Kishibe, M.; Yoshida, S.

In: Neuroscience, Vol. 148, No. 1, 10.08.2007, p. 175-187.

Research output: Contribution to journalArticle

Terayama, R, Bando, Y, Murakami, K, Kato, K, Kishibe, M & Yoshida, S 2007, 'Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury', Neuroscience, vol. 148, no. 1, pp. 175-187. https://doi.org/10.1016/j.neuroscience.2007.05.037
Terayama, R. ; Bando, Y. ; Murakami, K. ; Kato, K. ; Kishibe, M. ; Yoshida, S. / Neuropsin promotes oligodendrocyte death, demyelination and axonal degeneration after spinal cord injury. In: Neuroscience. 2007 ; Vol. 148, No. 1. pp. 175-187.
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