Neuroprotective effects of D-allose against retinal ischemia-reperfusion injury

Kazuyuki Hirooka, Osamu Miyamoto, Pan Jinming, Yinghua Du, Toshifumi Itano, Tetsuya Baba, Masaaki Tokuda, Fumio Shiraga

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

PURPOSE. To investigate the effect of D-allose, a rare sugar, against ischemia reperfusion injury in the rat retina. METHODS. Retinal ischemia was induced by increasing intraocular pressure to 130 mm Hg and maintaining that level for 45 minutes. Morphometric studies were performed to study the effect of D-allose on the histologic changes induced by ischemia in the rat retina. Glutamate release from the rat retina and intravitreal PO2 profiles were monitored during and after ischemia with a microdialysis biosensor and oxygen-sensitive microelectrodes. The release of hydrogen peroxide stained with diaminobenzidine hydrochloride was monitored by an in vitro retinal ischemia model. RESULTS. Seven days after the ischemia, significant reductions in both the number of ganglion cells and the thickness of the inner plexiform layer were observed. Pretreatment with Dallose significantly inhibited the ischemic injury of the inner retina. A large release of glutamate occurred during the ischemia. After the recirculation, glutamate levels were increased again and reached a maximum in approximately 20 minutes. The increases in extracellular glutamate during and after ischemia tend to be suppressed by administration of D-allose. D-Allose attenuated the increase in intravitreal PO2 during reperfusion. After the ischemia, production of hydrogen peroxide was detected within approximately 30 minutes. D-Allose suppressed the production of hydrogen peroxide. CONCLUSIONS. These results suggest that D-allose may protect neurons by decreasing extracellular glutamate and attenuating oxidative stress in ischemic insult.

Original languageEnglish
Pages (from-to)1653-1657
Number of pages5
JournalInvestigative Ophthalmology and Visual Science
Volume47
Issue number4
DOIs
Publication statusPublished - Apr 1 2006
Externally publishedYes

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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