Neuroprotective Effects of a Novel Antioxidant Mixture Twendee X in Mouse Stroke Model

Momoko Kusaki, Yasuyuki Ohta, Haruhiko Inufusa, Toru Yamashita, Ryuta Morihara, Yumiko Nakano, Xia Liu, Jingwei Shang, Feng Tian, Yusuke Fukui, Kota Sato, Mami Takemoto, Nozomi Hishikawa, Koji Abe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background Oxidative stress and inflammation are important aggravating factors in acute ischemic stroke. Methods In the present study, the neuroprotective effects of a novel antioxidant mixture Twendee X containing multiple antioxidative ingredients, such as coenzyme Q10, ascorbic acid, and cystine, were evaluated. After the pretreatment of a vehicle or Twendee X (20 mg/kg/d) for 14 days, mice were subjected to transient middle cerebral artery occlusion for 60 minutes and further treated with vehicle or Twendee X for 1 or 5 days. Results Twendee X administration reduced the infarct size, and reduced oxidative stress markers such as 8-hydroxy-2′-deoxyguanosine, 4-hydroxy-2-nonenal, and Nε-(carboxymethyl) lysine (one of advanced glycation end products), as well as inflammatory markers such as ionized calcium binding adapter molecule-1, tumor necrosis factor-α, and monocyte chemotactic protein-1. Conclusions In the present study, the neuroprotective effects of Twendee X were shown on transient middle cerebral artery occlusion mice via antioxidative and anti-inflammatory pathways, providing a potential of Twendee X as one preventive and therapeutic treatment.

Original languageEnglish
Pages (from-to)1191-1196
Number of pages6
JournalJournal of Stroke and Cerebrovascular Diseases
Volume26
Issue number6
DOIs
Publication statusPublished - Jun 1 2017

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coenzyme Q10
Middle Cerebral Artery Infarction
Neuroprotective Agents
Oxidative Stress
Antioxidants
Stroke
Advanced Glycosylation End Products
Cystine
Chemokine CCL2
Ascorbic Acid
Anti-Inflammatory Agents
Tumor Necrosis Factor-alpha
Inflammation
Calcium
Therapeutics
4-hydroxy-2-nonenal
8-oxo-7-hydrodeoxyguanosine
N(6)-carboxymethyllysine

Keywords

  • anti-inflammatory
  • antioxidative
  • Ischemic stroke
  • middle cerebral artery occlusion
  • mouse

ASJC Scopus subject areas

  • Surgery
  • Rehabilitation
  • Clinical Neurology
  • Cardiology and Cardiovascular Medicine

Cite this

Neuroprotective Effects of a Novel Antioxidant Mixture Twendee X in Mouse Stroke Model. / Kusaki, Momoko; Ohta, Yasuyuki; Inufusa, Haruhiko; Yamashita, Toru; Morihara, Ryuta; Nakano, Yumiko; Liu, Xia; Shang, Jingwei; Tian, Feng; Fukui, Yusuke; Sato, Kota; Takemoto, Mami; Hishikawa, Nozomi; Abe, Koji.

In: Journal of Stroke and Cerebrovascular Diseases, Vol. 26, No. 6, 01.06.2017, p. 1191-1196.

Research output: Contribution to journalArticle

Kusaki, Momoko ; Ohta, Yasuyuki ; Inufusa, Haruhiko ; Yamashita, Toru ; Morihara, Ryuta ; Nakano, Yumiko ; Liu, Xia ; Shang, Jingwei ; Tian, Feng ; Fukui, Yusuke ; Sato, Kota ; Takemoto, Mami ; Hishikawa, Nozomi ; Abe, Koji. / Neuroprotective Effects of a Novel Antioxidant Mixture Twendee X in Mouse Stroke Model. In: Journal of Stroke and Cerebrovascular Diseases. 2017 ; Vol. 26, No. 6. pp. 1191-1196.
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