Neuroprotective effect of D-Psicose on 6-hydroxydopamine-induced apoptosis in rat pheochromocytoma (PC12) cells

Maki K. Takata, Fuminori Yamaguchi, Koichi Nakanose, Yasuo Watanabe, Naoya Hatano, Ikuko Tsukamoto, Mitsuhiro Nagata, Ken Izumori, Masaaki Tokuda

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69 Citations (Scopus)


We evaluated the neuroprotective effects of D-psicose, one of the rare sugars, on 6-hydroxydopamine (6-OHDA)-induced apoptosis in catecholaminergic PC12 cells, the in vitro model of Parkinson's disease (PD). Apoptotic characteristics of PC12 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. The results showed that D-psicose at a concentration of 50 mM, exerted significant protective effects against the 6-OHDA (200 μM)-induced PC12 cell apoptosis, while other sugars had little or no protective effects. We have observed a significant increase in the level of intracellular glutathione after 24 h in 6-OHDA (200 μM) treated cells, while a decrease in the level was observed at 3 h and 6 h. Also, a synergistic exposure to D-psicose and 6-OHDA for 24 h showed a significant increase in intracellular glutathione level. Therefore, these results suggest that D-psicose may play a potential role as a neuroprotective agent in the treatment of neurodegenerative diseases by inducing an up-regulation of intracellular glutathione.

Original languageEnglish
Pages (from-to)511-516
Number of pages6
JournalJournal of Bioscience and Bioengineering
Issue number5
Publication statusPublished - 2005
Externally publishedYes


  • 6-hydroxydopamine (6-OHDA)
  • Antioxidant
  • Apoptosis
  • D-psicose
  • PC12 cells
  • Parkinson's disease (PD)
  • Rare sugars

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology


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