Neuroprotective effect of D-Psicose on 6-hydroxydopamine-induced apoptosis in rat pheochromocytoma (PC12) cells

Maki K. Takata; Fuminori Yamaguchi; Koichi Nakanose; Yasuo Watanabe; Naoya Hatano; Ikuko Tsukamoto; Mitsuhiro Nagata; Ken Izumori; Masaaki Tokuda

doi:10.1263/jbb.100.511

Neuroprotective effect of D-Psicose on 6-hydroxydopamine-induced apoptosis in rat pheochromocytoma (PC12) cells

Maki K. Takata, Fuminori Yamaguchi, Koichi Nakanose, Yasuo Watanabe, Naoya Hatano, Ikuko Tsukamoto, Mitsuhiro Nagata, Ken Izumori, Masaaki Tokuda

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Abstract

We evaluated the neuroprotective effects of D-psicose, one of the rare sugars, on 6-hydroxydopamine (6-OHDA)-induced apoptosis in catecholaminergic PC12 cells, the in vitro model of Parkinson's disease (PD). Apoptotic characteristics of PC12 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. The results showed that D-psicose at a concentration of 50 mM, exerted significant protective effects against the 6-OHDA (200 μM)-induced PC12 cell apoptosis, while other sugars had little or no protective effects. We have observed a significant increase in the level of intracellular glutathione after 24 h in 6-OHDA (200 μM) treated cells, while a decrease in the level was observed at 3 h and 6 h. Also, a synergistic exposure to D-psicose and 6-OHDA for 24 h showed a significant increase in intracellular glutathione level. Therefore, these results suggest that D-psicose may play a potential role as a neuroprotective agent in the treatment of neurodegenerative diseases by inducing an up-regulation of intracellular glutathione.

Original language	English
Pages (from-to)	511-516
Number of pages	6
Journal	Journal of Bioscience and Bioengineering
Volume	100
Issue number	5
DOIs	https://doi.org/10.1263/jbb.100.511
Publication status	Published - 2005
Externally published	Yes

Keywords

6-hydroxydopamine (6-OHDA)
Antioxidant
Apoptosis
D-psicose
PC12 cells
Parkinson's disease (PD)
Rare sugars

ASJC Scopus subject areas

Biotechnology
Bioengineering
Applied Microbiology and Biotechnology

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10.1263/jbb.100.511

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@article{6f443d9d6d4a418eac9a44a68e4c6e0d,

title = "Neuroprotective effect of D-Psicose on 6-hydroxydopamine-induced apoptosis in rat pheochromocytoma (PC12) cells",

abstract = "We evaluated the neuroprotective effects of D-psicose, one of the rare sugars, on 6-hydroxydopamine (6-OHDA)-induced apoptosis in catecholaminergic PC12 cells, the in vitro model of Parkinson's disease (PD). Apoptotic characteristics of PC12 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. The results showed that D-psicose at a concentration of 50 mM, exerted significant protective effects against the 6-OHDA (200 μM)-induced PC12 cell apoptosis, while other sugars had little or no protective effects. We have observed a significant increase in the level of intracellular glutathione after 24 h in 6-OHDA (200 μM) treated cells, while a decrease in the level was observed at 3 h and 6 h. Also, a synergistic exposure to D-psicose and 6-OHDA for 24 h showed a significant increase in intracellular glutathione level. Therefore, these results suggest that D-psicose may play a potential role as a neuroprotective agent in the treatment of neurodegenerative diseases by inducing an up-regulation of intracellular glutathione.",

keywords = "6-hydroxydopamine (6-OHDA), Antioxidant, Apoptosis, D-psicose, PC12 cells, Parkinson's disease (PD), Rare sugars",

author = "Takata, {Maki K.} and Fuminori Yamaguchi and Koichi Nakanose and Yasuo Watanabe and Naoya Hatano and Ikuko Tsukamoto and Mitsuhiro Nagata and Ken Izumori and Masaaki Tokuda",

note = "Funding Information: The authors thank Mr. Singh K. for proofreading the manuscript and to the group of rare sugar production for the supply of rare sugars used in this study. This study was supported in part by a grant of the “intellectual-cluster” from the Ministry of Education, Culture, Sports, Science and Technology of Japan.",

year = "2005",

doi = "10.1263/jbb.100.511",

language = "English",

volume = "100",

pages = "511--516",

journal = "Journal of Bioscience and Bioengineering",

issn = "1389-1723",

publisher = "The Society for Biotechnology, Japan",

number = "5",

}

TY - JOUR

T1 - Neuroprotective effect of D-Psicose on 6-hydroxydopamine-induced apoptosis in rat pheochromocytoma (PC12) cells

AU - Takata, Maki K.

AU - Yamaguchi, Fuminori

AU - Nakanose, Koichi

AU - Watanabe, Yasuo

AU - Hatano, Naoya

AU - Tsukamoto, Ikuko

AU - Nagata, Mitsuhiro

AU - Izumori, Ken

AU - Tokuda, Masaaki

N1 - Funding Information: The authors thank Mr. Singh K. for proofreading the manuscript and to the group of rare sugar production for the supply of rare sugars used in this study. This study was supported in part by a grant of the “intellectual-cluster” from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

PY - 2005

Y1 - 2005

N2 - We evaluated the neuroprotective effects of D-psicose, one of the rare sugars, on 6-hydroxydopamine (6-OHDA)-induced apoptosis in catecholaminergic PC12 cells, the in vitro model of Parkinson's disease (PD). Apoptotic characteristics of PC12 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. The results showed that D-psicose at a concentration of 50 mM, exerted significant protective effects against the 6-OHDA (200 μM)-induced PC12 cell apoptosis, while other sugars had little or no protective effects. We have observed a significant increase in the level of intracellular glutathione after 24 h in 6-OHDA (200 μM) treated cells, while a decrease in the level was observed at 3 h and 6 h. Also, a synergistic exposure to D-psicose and 6-OHDA for 24 h showed a significant increase in intracellular glutathione level. Therefore, these results suggest that D-psicose may play a potential role as a neuroprotective agent in the treatment of neurodegenerative diseases by inducing an up-regulation of intracellular glutathione.

AB - We evaluated the neuroprotective effects of D-psicose, one of the rare sugars, on 6-hydroxydopamine (6-OHDA)-induced apoptosis in catecholaminergic PC12 cells, the in vitro model of Parkinson's disease (PD). Apoptotic characteristics of PC12 cells were assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay and terminal deoxynucleotidyl transferase mediated dUTP nick end-labeling (TUNEL) assay. The results showed that D-psicose at a concentration of 50 mM, exerted significant protective effects against the 6-OHDA (200 μM)-induced PC12 cell apoptosis, while other sugars had little or no protective effects. We have observed a significant increase in the level of intracellular glutathione after 24 h in 6-OHDA (200 μM) treated cells, while a decrease in the level was observed at 3 h and 6 h. Also, a synergistic exposure to D-psicose and 6-OHDA for 24 h showed a significant increase in intracellular glutathione level. Therefore, these results suggest that D-psicose may play a potential role as a neuroprotective agent in the treatment of neurodegenerative diseases by inducing an up-regulation of intracellular glutathione.

KW - 6-hydroxydopamine (6-OHDA)

KW - Antioxidant

KW - Apoptosis

KW - D-psicose

KW - PC12 cells

KW - Parkinson's disease (PD)

KW - Rare sugars

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AN - SCOPUS:31844437972

VL - 100

SP - 511

EP - 516

JO - Journal of Bioscience and Bioengineering

JF - Journal of Bioscience and Bioengineering

SN - 1389-1723

IS - 5

ER -

Neuroprotective effect of D-Psicose on 6-hydroxydopamine-induced apoptosis in rat pheochromocytoma (PC12) cells

Abstract

Keywords

ASJC Scopus subject areas

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