Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress

Xiaowen Shi; Yasuyuki Ohta; Yumiko Nakano; Xia Liu; Koh Tadokoro; Tian Feng; Emi Nomura; Keiichiro Tsunoda; Ryo Sasaki; Namiko Matsumoto; Yosuke Osakada; Yuting Bian; Zhihong Bian; Yoshio Omote; Mami Takemoto; Nozomi Hishikawa; Toru Yamashita; Koji Abe

doi:10.1016/j.neures.2020.05.009

Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress

Xiaowen Shi, Yasuyuki Ohta, Yumiko Nakano, Xia Liu, Koh Tadokoro, Tian Feng, Emi Nomura, Keiichiro Tsunoda, Ryo Sasaki, Namiko Matsumoto, Yosuke Osakada, Yuting Bian, Zhihong Bian, Yoshio Omote, Mami Takemoto, Nozomi Hishikawa, Toru Yamashita, Koji Abe

Research output: Contribution to journal › Article › peer-review

Abstract

Cu-diacetyl-bis (N4-methylthiosemicarbazone) (CuATSM) has both anti-oxidative and anti-inflammatory activities, but its therapeutic efficacy for oxidative stress has not been thoroughly investigated in acute ischemic stroke. Here, the present study was designed to assess the efficacies of CuATSM in acute ischemic stroke by comparing with the standard neuroprotective reagent edaravone. Mice were subjected to transient middle cerebral occlusion (tMCAO) for 60 min, and then intravenously administrated with CuATSM (1.5 mg/kg) or edaravone (3 mg/kg) just after the reperfusion, and examined at 1 and 3 d. Compared with the vehicle group, CuATSM treatment decreased infarct volumes and oxidative stress at 3d after tMCAO, which was further enhanced by combined CuATSM + edaravone treatment as compared with single CuATSM group, but not improve neurobehaviors. The present study demonstrated that CuATSM showed strong antioxidative and neuroprotective effects in acute ischemic stroke, which was enhanced by the combination with edaravone.

Original language	English
Journal	Neuroscience Research
DOIs	https://doi.org/10.1016/j.neures.2020.05.009
Publication status	Accepted/In press - 2020

Keywords

CuATSM
Edaravone
Oxidative stress
Stroke
Transient middle cerebral artery occlusion

ASJC Scopus subject areas

Neuroscience(all)

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10.1016/j.neures.2020.05.009

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Shi, X., Ohta, Y., Nakano, Y., Liu, X., Tadokoro, K., Feng, T., Nomura, E., Tsunoda, K., Sasaki, R., Matsumoto, N., Osakada, Y., Bian, Y., Bian, Z., Omote, Y., Takemoto, M., Hishikawa, N., Yamashita, T., & Abe, K. (Accepted/In press). Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress. Neuroscience Research. https://doi.org/10.1016/j.neures.2020.05.009

Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress. / Shi, Xiaowen; Ohta, Yasuyuki; Nakano, Yumiko; Liu, Xia; Tadokoro, Koh; Feng, Tian; Nomura, Emi; Tsunoda, Keiichiro; Sasaki, Ryo; Matsumoto, Namiko; Osakada, Yosuke; Bian, Yuting; Bian, Zhihong; Omote, Yoshio ; Takemoto, Mami ; Hishikawa, Nozomi ; Yamashita, Toru ; Abe, Koji.

In: Neuroscience Research, 2020.

Research output: Contribution to journal › Article › peer-review

Shi, Xiaowen ; Ohta, Yasuyuki ; Nakano, Yumiko ; Liu, Xia ; Tadokoro, Koh ; Feng, Tian ; Nomura, Emi ; Tsunoda, Keiichiro ; Sasaki, Ryo ; Matsumoto, Namiko ; Osakada, Yosuke ; Bian, Yuting ; Bian, Zhihong ; Omote, Yoshio ; Takemoto, Mami ; Hishikawa, Nozomi ; Yamashita, Toru ; Abe, Koji. / Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress. In: Neuroscience Research. 2020.

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title = "Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress",

abstract = "Cu-diacetyl-bis (N4-methylthiosemicarbazone) (CuATSM) has both anti-oxidative and anti-inflammatory activities, but its therapeutic efficacy for oxidative stress has not been thoroughly investigated in acute ischemic stroke. Here, the present study was designed to assess the efficacies of CuATSM in acute ischemic stroke by comparing with the standard neuroprotective reagent edaravone. Mice were subjected to transient middle cerebral occlusion (tMCAO) for 60 min, and then intravenously administrated with CuATSM (1.5 mg/kg) or edaravone (3 mg/kg) just after the reperfusion, and examined at 1 and 3 d. Compared with the vehicle group, CuATSM treatment decreased infarct volumes and oxidative stress at 3d after tMCAO, which was further enhanced by combined CuATSM + edaravone treatment as compared with single CuATSM group, but not improve neurobehaviors. The present study demonstrated that CuATSM showed strong antioxidative and neuroprotective effects in acute ischemic stroke, which was enhanced by the combination with edaravone.",

keywords = "CuATSM, Edaravone, Oxidative stress, Stroke, Transient middle cerebral artery occlusion",

author = "Xiaowen Shi and Yasuyuki Ohta and Yumiko Nakano and Xia Liu and Koh Tadokoro and Tian Feng and Emi Nomura and Keiichiro Tsunoda and Ryo Sasaki and Namiko Matsumoto and Yosuke Osakada and Yuting Bian and Zhihong Bian and Yoshio Omote and Mami Takemoto and Nozomi Hishikawa and Toru Yamashita and Koji Abe",

note = "Funding Information: This work was partly supported by a Grant-in-Aid for Scientific Research (B) 17H0419619 , (C) 15K0931607 , 17H0419619 and 17K1082709 , and by Grants-in-Aid from the Research Committees (Kaji R, Toba K, and Tsuji S) from Japan Agency for Medical Research and development ( AMED ) 7211700176 , 7211700180 and 7211700095 . X. S. would like to thank Otsuka Toshimi Scholarship Foundation for a scholarship support (19-91). Publisher Copyright: {\textcopyright} 2020",

year = "2020",

doi = "10.1016/j.neures.2020.05.009",

language = "English",

journal = "Neuroscience Research",

issn = "0168-0102",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress

AU - Shi, Xiaowen

AU - Ohta, Yasuyuki

AU - Nakano, Yumiko

AU - Liu, Xia

AU - Tadokoro, Koh

AU - Feng, Tian

AU - Nomura, Emi

AU - Tsunoda, Keiichiro

AU - Sasaki, Ryo

AU - Matsumoto, Namiko

AU - Osakada, Yosuke

AU - Bian, Yuting

AU - Bian, Zhihong

AU - Omote, Yoshio

AU - Takemoto, Mami

AU - Hishikawa, Nozomi

AU - Yamashita, Toru

AU - Abe, Koji

N1 - Funding Information: This work was partly supported by a Grant-in-Aid for Scientific Research (B) 17H0419619 , (C) 15K0931607 , 17H0419619 and 17K1082709 , and by Grants-in-Aid from the Research Committees (Kaji R, Toba K, and Tsuji S) from Japan Agency for Medical Research and development ( AMED ) 7211700176 , 7211700180 and 7211700095 . X. S. would like to thank Otsuka Toshimi Scholarship Foundation for a scholarship support (19-91). Publisher Copyright: © 2020

PY - 2020

Y1 - 2020

N2 - Cu-diacetyl-bis (N4-methylthiosemicarbazone) (CuATSM) has both anti-oxidative and anti-inflammatory activities, but its therapeutic efficacy for oxidative stress has not been thoroughly investigated in acute ischemic stroke. Here, the present study was designed to assess the efficacies of CuATSM in acute ischemic stroke by comparing with the standard neuroprotective reagent edaravone. Mice were subjected to transient middle cerebral occlusion (tMCAO) for 60 min, and then intravenously administrated with CuATSM (1.5 mg/kg) or edaravone (3 mg/kg) just after the reperfusion, and examined at 1 and 3 d. Compared with the vehicle group, CuATSM treatment decreased infarct volumes and oxidative stress at 3d after tMCAO, which was further enhanced by combined CuATSM + edaravone treatment as compared with single CuATSM group, but not improve neurobehaviors. The present study demonstrated that CuATSM showed strong antioxidative and neuroprotective effects in acute ischemic stroke, which was enhanced by the combination with edaravone.

AB - Cu-diacetyl-bis (N4-methylthiosemicarbazone) (CuATSM) has both anti-oxidative and anti-inflammatory activities, but its therapeutic efficacy for oxidative stress has not been thoroughly investigated in acute ischemic stroke. Here, the present study was designed to assess the efficacies of CuATSM in acute ischemic stroke by comparing with the standard neuroprotective reagent edaravone. Mice were subjected to transient middle cerebral occlusion (tMCAO) for 60 min, and then intravenously administrated with CuATSM (1.5 mg/kg) or edaravone (3 mg/kg) just after the reperfusion, and examined at 1 and 3 d. Compared with the vehicle group, CuATSM treatment decreased infarct volumes and oxidative stress at 3d after tMCAO, which was further enhanced by combined CuATSM + edaravone treatment as compared with single CuATSM group, but not improve neurobehaviors. The present study demonstrated that CuATSM showed strong antioxidative and neuroprotective effects in acute ischemic stroke, which was enhanced by the combination with edaravone.

KW - CuATSM

KW - Edaravone

KW - Oxidative stress

KW - Stroke

KW - Transient middle cerebral artery occlusion

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U2 - 10.1016/j.neures.2020.05.009

DO - 10.1016/j.neures.2020.05.009

M3 - Article

C2 - 32461139

AN - SCOPUS:85085922054

JO - Neuroscience Research

JF - Neuroscience Research

SN - 0168-0102

ER -

Neuroprotective effect of CuATSM in mice stroke model by ameliorating oxidative stress

Abstract

Keywords

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