Neurons with Strongly Negative-Charged Surface-Coats in Adult Rat Brain as Detected by Staining with Cationic Iron Colloid

Takuro Murakami, Takehito Taguchi, Aiji Ohtsuka, Akio Kikuta

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Light microscopy of tissue sections stained either with cationic iron colloid (pH 1.0-2.0) and nuclear fast red or with this colloid and thionin showed that the adult rat brain contains a considerable number of neurons which are strongly negative-charged by being coated with sulfated proteoglycans such as chondroitin sulfates. These neurons are distributed mainly in the cerebral cortex, hippocampus, zona incerta, medial and lateral cerebellar nuclei, ventral pontine nuclei and certain other areas. In the hippocampal formation, the strongly negative-charged cells seem identical with the GABAergic inhibitory interneurons reactive to the lectin Vicia villosa agglutinin. Neurons, including the GABAergic Purkinje's cells, of the cerebellar cortex showed no reaction to our cationic iron colloid at pH values of 1.0-2.0. Many non-GABAergic pyramidal cells in the lamina ganglionalis of cerebral cortex and many non-GABAergic large neurons of the ventral pontine nuclei were highly reactive to our colloid at pH values of 1.0-2.0. This suggests that our cationic iron colloid at pH values of 1.0-2.0 mainly stains certain subtypes of GABAergic neurons as well as some non-GABAergic neurons projecting long associational or commissural fibers.

Original languageEnglish
Pages (from-to)13-21
Number of pages9
Journalarchives of histology and cytology
Volume56
Issue number1
DOIs
Publication statusPublished - Jan 1 1993

ASJC Scopus subject areas

  • Histology

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