Nerve-dependent and -independent events in blastema formation during Xenopus froglet limb regeneration

Makoto Suzuki, Akira Sato, Hiroyuki Ide, Koji Tamura

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Blastema formation, the initial stage of epimorphic limb regeneration in amphibians, is an essential process to produce regenerates. In our study on nerve dependency of blastema formation, we used forelimb of Xenopus laevis froglets as a system and applied some histological and molecular approaches in order to determine early events during blastema formation. We also investigated the lateral wound healing in comparison to blastema formation in limb regeneration. Our study confirmed at the molecular level that there are nerve-dependent and -independent events during blastema formation after limb amputation, Tbx5 and Prx1, reliable markers of initiation of limb regeneration, that start to be expressed independently of nerve supply, although their expressions cannot be maintained without nerve supply. We also found that cell proliferation activity, cell survival and expression of Fgf8, Fgf10 and Msx1 in the blastema were affected by denervation, suggesting that these events specific for blastema outgrowth are controlled by the nerve supply. Wound healing, which is thought to be categorized into tissue regeneration, shares some nerve-independent events with epimorphic limb regeneration, although the healing process results in simple restoration of wounded tissue. Overall, our results demonstrate that dedifferentiated blastemal cells formed at the initial phase of limb regeneration must enter the nerve-dependent epimorphic phase for further processes, including blastema outgrowth, and that failure of entry results in a simple redifferentiation as tissue regeneration.

Original languageEnglish
Pages (from-to)361-375
Number of pages15
JournalDevelopmental Biology
Volume286
Issue number1
DOIs
Publication statusPublished - Oct 1 2005
Externally publishedYes

Fingerprint

Xenopus
Regeneration
Extremities
Wound Healing
Forelimb
Xenopus laevis
Amphibians
Denervation
Amputation
Cell Survival
Cell Proliferation

Keywords

  • Blastema
  • Fgf10
  • Fgf8
  • Limb regeneration
  • Msx1
  • Nerve dependency
  • Prx1
  • Tbx5
  • Wound healing
  • Xenopus

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Nerve-dependent and -independent events in blastema formation during Xenopus froglet limb regeneration. / Suzuki, Makoto; Sato, Akira; Ide, Hiroyuki; Tamura, Koji.

In: Developmental Biology, Vol. 286, No. 1, 01.10.2005, p. 361-375.

Research output: Contribution to journalArticle

Suzuki, Makoto ; Sato, Akira ; Ide, Hiroyuki ; Tamura, Koji. / Nerve-dependent and -independent events in blastema formation during Xenopus froglet limb regeneration. In: Developmental Biology. 2005 ; Vol. 286, No. 1. pp. 361-375.
@article{04e1e5676f934dfd95274001051ddb89,
title = "Nerve-dependent and -independent events in blastema formation during Xenopus froglet limb regeneration",
abstract = "Blastema formation, the initial stage of epimorphic limb regeneration in amphibians, is an essential process to produce regenerates. In our study on nerve dependency of blastema formation, we used forelimb of Xenopus laevis froglets as a system and applied some histological and molecular approaches in order to determine early events during blastema formation. We also investigated the lateral wound healing in comparison to blastema formation in limb regeneration. Our study confirmed at the molecular level that there are nerve-dependent and -independent events during blastema formation after limb amputation, Tbx5 and Prx1, reliable markers of initiation of limb regeneration, that start to be expressed independently of nerve supply, although their expressions cannot be maintained without nerve supply. We also found that cell proliferation activity, cell survival and expression of Fgf8, Fgf10 and Msx1 in the blastema were affected by denervation, suggesting that these events specific for blastema outgrowth are controlled by the nerve supply. Wound healing, which is thought to be categorized into tissue regeneration, shares some nerve-independent events with epimorphic limb regeneration, although the healing process results in simple restoration of wounded tissue. Overall, our results demonstrate that dedifferentiated blastemal cells formed at the initial phase of limb regeneration must enter the nerve-dependent epimorphic phase for further processes, including blastema outgrowth, and that failure of entry results in a simple redifferentiation as tissue regeneration.",
keywords = "Blastema, Fgf10, Fgf8, Limb regeneration, Msx1, Nerve dependency, Prx1, Tbx5, Wound healing, Xenopus",
author = "Makoto Suzuki and Akira Sato and Hiroyuki Ide and Koji Tamura",
year = "2005",
month = "10",
day = "1",
doi = "10.1016/j.ydbio.2005.08.021",
language = "English",
volume = "286",
pages = "361--375",
journal = "Developmental Biology",
issn = "0012-1606",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Nerve-dependent and -independent events in blastema formation during Xenopus froglet limb regeneration

AU - Suzuki, Makoto

AU - Sato, Akira

AU - Ide, Hiroyuki

AU - Tamura, Koji

PY - 2005/10/1

Y1 - 2005/10/1

N2 - Blastema formation, the initial stage of epimorphic limb regeneration in amphibians, is an essential process to produce regenerates. In our study on nerve dependency of blastema formation, we used forelimb of Xenopus laevis froglets as a system and applied some histological and molecular approaches in order to determine early events during blastema formation. We also investigated the lateral wound healing in comparison to blastema formation in limb regeneration. Our study confirmed at the molecular level that there are nerve-dependent and -independent events during blastema formation after limb amputation, Tbx5 and Prx1, reliable markers of initiation of limb regeneration, that start to be expressed independently of nerve supply, although their expressions cannot be maintained without nerve supply. We also found that cell proliferation activity, cell survival and expression of Fgf8, Fgf10 and Msx1 in the blastema were affected by denervation, suggesting that these events specific for blastema outgrowth are controlled by the nerve supply. Wound healing, which is thought to be categorized into tissue regeneration, shares some nerve-independent events with epimorphic limb regeneration, although the healing process results in simple restoration of wounded tissue. Overall, our results demonstrate that dedifferentiated blastemal cells formed at the initial phase of limb regeneration must enter the nerve-dependent epimorphic phase for further processes, including blastema outgrowth, and that failure of entry results in a simple redifferentiation as tissue regeneration.

AB - Blastema formation, the initial stage of epimorphic limb regeneration in amphibians, is an essential process to produce regenerates. In our study on nerve dependency of blastema formation, we used forelimb of Xenopus laevis froglets as a system and applied some histological and molecular approaches in order to determine early events during blastema formation. We also investigated the lateral wound healing in comparison to blastema formation in limb regeneration. Our study confirmed at the molecular level that there are nerve-dependent and -independent events during blastema formation after limb amputation, Tbx5 and Prx1, reliable markers of initiation of limb regeneration, that start to be expressed independently of nerve supply, although their expressions cannot be maintained without nerve supply. We also found that cell proliferation activity, cell survival and expression of Fgf8, Fgf10 and Msx1 in the blastema were affected by denervation, suggesting that these events specific for blastema outgrowth are controlled by the nerve supply. Wound healing, which is thought to be categorized into tissue regeneration, shares some nerve-independent events with epimorphic limb regeneration, although the healing process results in simple restoration of wounded tissue. Overall, our results demonstrate that dedifferentiated blastemal cells formed at the initial phase of limb regeneration must enter the nerve-dependent epimorphic phase for further processes, including blastema outgrowth, and that failure of entry results in a simple redifferentiation as tissue regeneration.

KW - Blastema

KW - Fgf10

KW - Fgf8

KW - Limb regeneration

KW - Msx1

KW - Nerve dependency

KW - Prx1

KW - Tbx5

KW - Wound healing

KW - Xenopus

UR - http://www.scopus.com/inward/record.url?scp=25844467734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=25844467734&partnerID=8YFLogxK

U2 - 10.1016/j.ydbio.2005.08.021

DO - 10.1016/j.ydbio.2005.08.021

M3 - Article

C2 - 16154125

AN - SCOPUS:25844467734

VL - 286

SP - 361

EP - 375

JO - Developmental Biology

JF - Developmental Biology

SN - 0012-1606

IS - 1

ER -