Nephritogenicity and α-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane

Syarifuddin Rauf, Megumi Kagawa, Yumiko Kishiro, Satoko Inoue, Ichiro Naito, Toshitaka Oohashi, Manabu Sugimoto, Yoshifumi Ninomiya, Yoshikazu Sado

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Nephritogenicity (anti-GBM-nephritis-inducing activity) and α-chain composition of globular-domain (NC1) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different α chains of type IV collagen. A purified nephritogenic fraction from renal BM contained α1-α6(IV)NC1, whereas a non-nephritogenic fraction contained only α1-α2(IV)NC1. Penal and pulmonary NC1 had strong nephritogenic activity; placental NC1 had weak activity. The renal and pulmonary fractions contained α1-α6(IV)NC1, and the placental fraction had a large amount of α1-α2(IV)NC1 and a very small amount of α3-α6(IV)NC1. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained α1-α5(IV) chains, but not the α6(IV) chain. The absence of α6(IV) chain in glomerular EM in bovine and in humans indicates that α6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of α3-α5 (IV)NC1.

Original languageEnglish
Pages (from-to)281-288
Number of pages8
JournalVirchows Archiv
Volume428
Issue number4-5
Publication statusPublished - Aug 23 1996

Keywords

  • Glomerular basement membrane
  • Glomerulonephritis
  • Goodpasture's syndrome
  • Monoclonal antibody
  • NC1
  • Nephritogenic antigen
  • Type IV collagen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Molecular Biology
  • Cell Biology

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