Abstract
Nephritogenicity (anti-GBM-nephritis-inducing activity) and α-chain composition of globular-domain (NC1) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different α chains of type IV collagen. A purified nephritogenic fraction from renal BM contained α1-α6(IV)NC1, whereas a non-nephritogenic fraction contained only α1-α2(IV)NC1. Penal and pulmonary NC1 had strong nephritogenic activity; placental NC1 had weak activity. The renal and pulmonary fractions contained α1-α6(IV)NC1, and the placental fraction had a large amount of α1-α2(IV)NC1 and a very small amount of α3-α6(IV)NC1. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained α1-α5(IV) chains, but not the α6(IV) chain. The absence of α6(IV) chain in glomerular EM in bovine and in humans indicates that α6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of α3-α5 (IV)NC1.
| Original language | English |
|---|---|
| Pages (from-to) | 281-288 |
| Number of pages | 8 |
| Journal | Virchows Archiv |
| Volume | 428 |
| Issue number | 4-5 |
| Publication status | Published - Aug 23 1996 |
Keywords
- Glomerular basement membrane
- Glomerulonephritis
- Goodpasture's syndrome
- Monoclonal antibody
- NC1
- Nephritogenic antigen
- Type IV collagen
ASJC Scopus subject areas
- Pathology and Forensic Medicine
- Molecular Biology
- Cell Biology