Abstract
Nephritogenicity (anti-GBM-nephritis-inducing activity) and α-chain composition of globular-domain (NC1) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different α chains of type IV collagen. A purified nephritogenic fraction from renal BM contained α1-α6(IV)NC1, whereas a non-nephritogenic fraction contained only α1-α2(IV)NC1. Penal and pulmonary NC1 had strong nephritogenic activity; placental NC1 had weak activity. The renal and pulmonary fractions contained α1-α6(IV)NC1, and the placental fraction had a large amount of α1-α2(IV)NC1 and a very small amount of α3-α6(IV)NC1. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained α1-α5(IV) chains, but not the α6(IV) chain. The absence of α6(IV) chain in glomerular EM in bovine and in humans indicates that α6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of α3-α5 (IV)NC1.
| Original language | English |
|---|---|
| Pages (from-to) | 281-288 |
| Number of pages | 8 |
| Journal | Virchows Archiv |
| Volume | 428 |
| Issue number | 4-5 |
| Publication status | Published - 1996 |
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Keywords
- Glomerular basement membrane
- Glomerulonephritis
- Goodpasture's syndrome
- Monoclonal antibody
- NC1
- Nephritogenic antigen
- Type IV collagen
ASJC Scopus subject areas
- Pathology and Forensic Medicine
Cite this
Nephritogenicity and α-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane. / Rauf, Syarifuddin; Kagawa, Megumi; Kishiro, Yumiko; Inoue, Satoko; Naito, Ichiro; Oohashi, Toshitaka; Sugimoto, Manabu; Ninomiya, Yoshifumi; Sado, Yoshikazu.
In: Virchows Archiv, Vol. 428, No. 4-5, 1996, p. 281-288.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Nephritogenicity and α-chain composition of NC1 fractions of type IV collagen from bovine renal basement membrane
AU - Rauf, Syarifuddin
AU - Kagawa, Megumi
AU - Kishiro, Yumiko
AU - Inoue, Satoko
AU - Naito, Ichiro
AU - Oohashi, Toshitaka
AU - Sugimoto, Manabu
AU - Ninomiya, Yoshifumi
AU - Sado, Yoshikazu
PY - 1996
Y1 - 1996
N2 - Nephritogenicity (anti-GBM-nephritis-inducing activity) and α-chain composition of globular-domain (NC1) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different α chains of type IV collagen. A purified nephritogenic fraction from renal BM contained α1-α6(IV)NC1, whereas a non-nephritogenic fraction contained only α1-α2(IV)NC1. Penal and pulmonary NC1 had strong nephritogenic activity; placental NC1 had weak activity. The renal and pulmonary fractions contained α1-α6(IV)NC1, and the placental fraction had a large amount of α1-α2(IV)NC1 and a very small amount of α3-α6(IV)NC1. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained α1-α5(IV) chains, but not the α6(IV) chain. The absence of α6(IV) chain in glomerular EM in bovine and in humans indicates that α6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of α3-α5 (IV)NC1.
AB - Nephritogenicity (anti-GBM-nephritis-inducing activity) and α-chain composition of globular-domain (NC1) fractions of type IV collagen from bovine renal, pulmonary, and placental basement membranes (BMs) was examined by injecting these fractions with adjuvant into WKY/NCrj rats and by Western blotting using epitope-defined monoclonal antibodies to the six different α chains of type IV collagen. A purified nephritogenic fraction from renal BM contained α1-α6(IV)NC1, whereas a non-nephritogenic fraction contained only α1-α2(IV)NC1. Penal and pulmonary NC1 had strong nephritogenic activity; placental NC1 had weak activity. The renal and pulmonary fractions contained α1-α6(IV)NC1, and the placental fraction had a large amount of α1-α2(IV)NC1 and a very small amount of α3-α6(IV)NC1. Immunohistochemical study of bovine renal BM with the monoclonal antibodies revealed that bovine glomerular BM contained α1-α5(IV) chains, but not the α6(IV) chain. The absence of α6(IV) chain in glomerular EM in bovine and in humans indicates that α6(IV) chain is not a target antigen of anti-GBM nephritis. Nephritogenicity is apparently a property of α3-α5 (IV)NC1.
KW - Glomerular basement membrane
KW - Glomerulonephritis
KW - Goodpasture's syndrome
KW - Monoclonal antibody
KW - NC1
KW - Nephritogenic antigen
KW - Type IV collagen
UR - http://www.scopus.com/inward/record.url?scp=0029834459&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029834459&partnerID=8YFLogxK
M3 - Article
VL - 428
SP - 281
EP - 288
JO - Virchows Archiv
JF - Virchows Archiv
SN - 0945-6317
IS - 4-5
ER -