Neoplastic thymic epithelial cells of human thymoma support T cell development from CD4-CD8- cells to CD4+CD8+ cells in vitro

M. Inoue, Y. Fujii, M. Okumura, Y. Takeuchi, H. Shiono, S. Miyoshi, H. Matsuda, R. Shirakura

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Human thymoma is a thymic epithelial cell tumour which often contains a large number of immature T cells and is frequently associated with autoimmune diseases. Since thymic epithelial cells play key roles in the development and selection of T cells in the normal thymus, we hypothesized that the neoplastic thymic epithelial cells of thymoma may support T cell differentiation in the tumour. We characterized CD4-CD8- cells in thymoma and applied an in vitro reconstitution culture system using the CD4-CD8- cells and the neoplastic epithelial cells isolated from thymoma. CD34, a stem cell marker, was expressed on 29.9 ± 12.2% of CD4-CD8- cells in thymoma. TCRγδ was expressed on 27.4 ± 15.1% of CD4-CD8- cells and CD19, a B cell marker, was expressed on 14-1 ± 23.1% of CD4-CD8- cells. CD4-CD8- cells expressed both IL-7R α-chain and common γ-chain. Purified CD4-CD8- cells from thymomas were cultured with the neoplastic epithelial cells, and their differentiation into CD4+CD8+ cells via CD4 single-positive intermediates was observed within 9 days' co-culture in the presence of recombinant IL-7. Furthermore, we examined the reconstitution culture using CD34+CD4-CD8- cells purified from normal infant thymus. The CD34+CD4-CD8-cells in normal thymus also differentiated to CD4+CD8+ cells in the allogeneic co-culture with the neoplastic epithelial cells of thymoma. These results indicate that the tumour cells of thymoma retain the function of thymic epithelial cells and can induce differentiation of T cells in thymoma.

Original languageEnglish
Pages (from-to)419-426
Number of pages8
JournalClinical and Experimental Immunology
Volume112
Issue number3
DOIs
Publication statusPublished - 1998

Keywords

  • Human
  • T cell differentiation
  • Thymic epithelial cell
  • Thymoma

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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