Neonicotinoid resistance and cDNA sequences of nicotinic acetylcholine receptor subunits of the western flower thrips Frankliniella occidentalis (Thysanoptera: Thripidae)

Chieka Minakuchi, Yurika Inano, Xueyan Shi, Dunlun Song, Youjun Zhang, Ken Miura, Tadashi Miyata, Xiwu Gao, Toshiharu Tanaka, Shoji Sonoda

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The western flower thrips, Frankliniella occidentalis (Pergande), is difficult to control because of high insecticide resistance. In this study, susceptibility to major insecticides was examined in two Japanese strains (H-1 and KC) and a Chinese strain (BJ) using a leaf-dipping method. All three strains were resistant to permethrin and acetamiprid at agriculturally recommended doses. The median lethal concentration (LC50) for acetamiprid was 1720 ppm in strain H-1, 4780 ppm in strain KC and >6680 ppm in strain BJ. In the presence of piperonyl butoxide, an inhibitor of cytochrome P450 monooxygenases, the LC50 for acetamiprid was 312 ppm in strain H-1, 837 ppm in strain KC and 1250 ppm in strain BJ. These results suggested that metabolism by cytochrome P450 monooxygenases is involved in acetamiprid resistance in these strains, though other factors also seem to play a role. Furthermore, cDNA cloning of the nicotinic acetylcholine receptor (nAChR) subunits was performed using degenerate primers, and the presence or absence of a point mutation in nAChR β1 was confirmed. The R81T mutation that had been reported in Myzus persicae (Sulzer) nAChR β1 was not found in F. occidentalis strains tested in this study.

Original languageEnglish
Pages (from-to)507-513
Number of pages7
JournalApplied Entomology and Zoology
Volume48
Issue number4
DOIs
Publication statusPublished - Nov 2013

Fingerprint

neonicotinoid insecticides
Frankliniella occidentalis
cholinergic receptors
Thripidae
Thysanoptera
acetamiprid
cytochrome P-450
lethal concentration 50
piperonyl butoxide
insecticide resistance
permethrin
Myzus persicae
dipping
point mutation
lethal genes
molecular cloning
insecticides

Keywords

  • Frankliniella occidentalis
  • Neonicotinoids
  • Nicotinic acetylcholine receptor
  • Piperonyl butoxide

ASJC Scopus subject areas

  • Insect Science

Cite this

Neonicotinoid resistance and cDNA sequences of nicotinic acetylcholine receptor subunits of the western flower thrips Frankliniella occidentalis (Thysanoptera : Thripidae). / Minakuchi, Chieka; Inano, Yurika; Shi, Xueyan; Song, Dunlun; Zhang, Youjun; Miura, Ken; Miyata, Tadashi; Gao, Xiwu; Tanaka, Toshiharu; Sonoda, Shoji.

In: Applied Entomology and Zoology, Vol. 48, No. 4, 11.2013, p. 507-513.

Research output: Contribution to journalArticle

Minakuchi, Chieka ; Inano, Yurika ; Shi, Xueyan ; Song, Dunlun ; Zhang, Youjun ; Miura, Ken ; Miyata, Tadashi ; Gao, Xiwu ; Tanaka, Toshiharu ; Sonoda, Shoji. / Neonicotinoid resistance and cDNA sequences of nicotinic acetylcholine receptor subunits of the western flower thrips Frankliniella occidentalis (Thysanoptera : Thripidae). In: Applied Entomology and Zoology. 2013 ; Vol. 48, No. 4. pp. 507-513.
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