Negative regulation of TIMP1 is mediated by transcription factor TWIST1

Hirohiko Okamura, Kaya Yoshida, Tatsuji Haneji

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

TWIST1 is involved in tumor invasion and metastasis by promoting epithelial-to-mesenchymal transition. However, the molecular target of TWIST1 involving this mechanism is poorly understood. To identify the TWIST1-regulated genes, we established the Saos-2 cells stably expressing TWIST1 gene by transfecting TWIST1 cDNA into the cells and performed a differential display approach by using annealing control primers. Here, we report that one of the genes that were down-regulated in TWIST1 expressing cells is predicted to be TIMP1. TIMP1 has been reported as the naturally occurring specific inhibitor of matrix metalloproteinases (MMPs). Overexpression of TWIST1 gene suppressed the expression of TIMP1 mRNA but had no effect on TIMP2 and MMP-2 expression, as determined by semi-quantitative RT-PCR. We showed that TWIST1 was up-regulated in SCCBHY cells, which have a strong capacity of invasion into mandibular bone compared with SCCHN cells. Our present results suggest that TWIST1 is involved in tumor invasion by regulating the expression of TIMP1.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalInternational Journal of Oncology
Volume35
Issue number1
DOIs
Publication statusPublished - 2009
Externally publishedYes

Fingerprint

Transcription Factors
Genes
Epithelial-Mesenchymal Transition
Matrix Metalloproteinase Inhibitors
Matrix Metalloproteinase 2
Neoplasms
Complementary DNA
Neoplasm Metastasis
Gene Expression
Bone and Bones
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Differential display
  • Matrix metalloproteinases
  • Saos-2
  • Squamous carcinoma cell
  • Tumor invasion

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Negative regulation of TIMP1 is mediated by transcription factor TWIST1. / Okamura, Hirohiko; Yoshida, Kaya; Haneji, Tatsuji.

In: International Journal of Oncology, Vol. 35, No. 1, 2009, p. 181-186.

Research output: Contribution to journalArticle

Okamura, Hirohiko ; Yoshida, Kaya ; Haneji, Tatsuji. / Negative regulation of TIMP1 is mediated by transcription factor TWIST1. In: International Journal of Oncology. 2009 ; Vol. 35, No. 1. pp. 181-186.
@article{e1a6356f0f934f0c9d4f2f7020ad82c1,
title = "Negative regulation of TIMP1 is mediated by transcription factor TWIST1",
abstract = "TWIST1 is involved in tumor invasion and metastasis by promoting epithelial-to-mesenchymal transition. However, the molecular target of TWIST1 involving this mechanism is poorly understood. To identify the TWIST1-regulated genes, we established the Saos-2 cells stably expressing TWIST1 gene by transfecting TWIST1 cDNA into the cells and performed a differential display approach by using annealing control primers. Here, we report that one of the genes that were down-regulated in TWIST1 expressing cells is predicted to be TIMP1. TIMP1 has been reported as the naturally occurring specific inhibitor of matrix metalloproteinases (MMPs). Overexpression of TWIST1 gene suppressed the expression of TIMP1 mRNA but had no effect on TIMP2 and MMP-2 expression, as determined by semi-quantitative RT-PCR. We showed that TWIST1 was up-regulated in SCCBHY cells, which have a strong capacity of invasion into mandibular bone compared with SCCHN cells. Our present results suggest that TWIST1 is involved in tumor invasion by regulating the expression of TIMP1.",
keywords = "Differential display, Matrix metalloproteinases, Saos-2, Squamous carcinoma cell, Tumor invasion",
author = "Hirohiko Okamura and Kaya Yoshida and Tatsuji Haneji",
year = "2009",
doi = "10.3892/ijo_00000327",
language = "English",
volume = "35",
pages = "181--186",
journal = "International Journal of Oncology",
issn = "1019-6439",
publisher = "Spandidos Publications",
number = "1",

}

TY - JOUR

T1 - Negative regulation of TIMP1 is mediated by transcription factor TWIST1

AU - Okamura, Hirohiko

AU - Yoshida, Kaya

AU - Haneji, Tatsuji

PY - 2009

Y1 - 2009

N2 - TWIST1 is involved in tumor invasion and metastasis by promoting epithelial-to-mesenchymal transition. However, the molecular target of TWIST1 involving this mechanism is poorly understood. To identify the TWIST1-regulated genes, we established the Saos-2 cells stably expressing TWIST1 gene by transfecting TWIST1 cDNA into the cells and performed a differential display approach by using annealing control primers. Here, we report that one of the genes that were down-regulated in TWIST1 expressing cells is predicted to be TIMP1. TIMP1 has been reported as the naturally occurring specific inhibitor of matrix metalloproteinases (MMPs). Overexpression of TWIST1 gene suppressed the expression of TIMP1 mRNA but had no effect on TIMP2 and MMP-2 expression, as determined by semi-quantitative RT-PCR. We showed that TWIST1 was up-regulated in SCCBHY cells, which have a strong capacity of invasion into mandibular bone compared with SCCHN cells. Our present results suggest that TWIST1 is involved in tumor invasion by regulating the expression of TIMP1.

AB - TWIST1 is involved in tumor invasion and metastasis by promoting epithelial-to-mesenchymal transition. However, the molecular target of TWIST1 involving this mechanism is poorly understood. To identify the TWIST1-regulated genes, we established the Saos-2 cells stably expressing TWIST1 gene by transfecting TWIST1 cDNA into the cells and performed a differential display approach by using annealing control primers. Here, we report that one of the genes that were down-regulated in TWIST1 expressing cells is predicted to be TIMP1. TIMP1 has been reported as the naturally occurring specific inhibitor of matrix metalloproteinases (MMPs). Overexpression of TWIST1 gene suppressed the expression of TIMP1 mRNA but had no effect on TIMP2 and MMP-2 expression, as determined by semi-quantitative RT-PCR. We showed that TWIST1 was up-regulated in SCCBHY cells, which have a strong capacity of invasion into mandibular bone compared with SCCHN cells. Our present results suggest that TWIST1 is involved in tumor invasion by regulating the expression of TIMP1.

KW - Differential display

KW - Matrix metalloproteinases

KW - Saos-2

KW - Squamous carcinoma cell

KW - Tumor invasion

UR - http://www.scopus.com/inward/record.url?scp=67649449918&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649449918&partnerID=8YFLogxK

U2 - 10.3892/ijo_00000327

DO - 10.3892/ijo_00000327

M3 - Article

C2 - 19513566

AN - SCOPUS:67649449918

VL - 35

SP - 181

EP - 186

JO - International Journal of Oncology

JF - International Journal of Oncology

SN - 1019-6439

IS - 1

ER -