Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis

Nasima Akter, Eiji Okuma, Muhammad Abdus Sobahan, Misugi Uraji, Shintaro Munemasa, Yoshimasa Nakamura, Izumi Mori, Yoshiyuki Murata

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Glutathione (GSH) has been shown to negatively regulate methyl jasmonate (MeJA)-induced stomatal closure. We investigated the roles of GSH in MeJA signaling in guard cells using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase. MeJA-induced stomatal closure and decreased GSH contents in guard cells. Decreasing GSH by the cad2-1 mutation enhanced MeJA-induced stomatal closure. Depletion of GSH by the cad2-1 mutation or increment of GSH by GSH monoethyl ester did not affect either MeJA-induced production of reactive oxygen species (ROS) or MeJA-induced cytosolic alkalization in guard cells. MeJA and abscisic acid (ABA) induced stomatal closure and GSH depletion in atrbohD and atrbohF single mutants but not in the atrbohD atrbohF double mutant. Moreover, exogenous hydrogen peroxide induced stomatal closure but did not deplete GSH in guard cells. These results indicate that GSH affects MeJA signaling as well as ABA signaling and that GSH negatively regulates a signal component other than ROS production and cytosolic alkalization in MeJA signal pathway of Arabidopsis guard cells.

Original languageEnglish
Pages (from-to)208-215
Number of pages8
JournalJournal of Plant Growth Regulation
Volume32
Issue number1
DOIs
Publication statusPublished - 2013

Fingerprint

methyl jasmonate
glutathione
Arabidopsis
guard cells
alkalinization
mutants
abscisic acid
reactive oxygen species
mutation
ligases
hydrogen peroxide
signal transduction
esters
biosynthesis
enzymes

Keywords

  • Cytosolic alkalization
  • Glutathione
  • Guard cells
  • Methyl jasmonate
  • Reactive oxygen species

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science

Cite this

Negative Regulation of Methyl Jasmonate-Induced Stomatal Closure by Glutathione in Arabidopsis. / Akter, Nasima; Okuma, Eiji; Sobahan, Muhammad Abdus; Uraji, Misugi; Munemasa, Shintaro; Nakamura, Yoshimasa; Mori, Izumi; Murata, Yoshiyuki.

In: Journal of Plant Growth Regulation, Vol. 32, No. 1, 2013, p. 208-215.

Research output: Contribution to journalArticle

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AB - Glutathione (GSH) has been shown to negatively regulate methyl jasmonate (MeJA)-induced stomatal closure. We investigated the roles of GSH in MeJA signaling in guard cells using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase. MeJA-induced stomatal closure and decreased GSH contents in guard cells. Decreasing GSH by the cad2-1 mutation enhanced MeJA-induced stomatal closure. Depletion of GSH by the cad2-1 mutation or increment of GSH by GSH monoethyl ester did not affect either MeJA-induced production of reactive oxygen species (ROS) or MeJA-induced cytosolic alkalization in guard cells. MeJA and abscisic acid (ABA) induced stomatal closure and GSH depletion in atrbohD and atrbohF single mutants but not in the atrbohD atrbohF double mutant. Moreover, exogenous hydrogen peroxide induced stomatal closure but did not deplete GSH in guard cells. These results indicate that GSH affects MeJA signaling as well as ABA signaling and that GSH negatively regulates a signal component other than ROS production and cytosolic alkalization in MeJA signal pathway of Arabidopsis guard cells.

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