Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis

Eiji Okuma, Md Sarwar Jahan, Shintaro Munemasa, Mohammad Anowar Hossain, Daichi Muroyama, Mohammad Mahbub Islam, Ken'ichi Ogawa, Megumi Watanabe-Sugimoto, Yoshimasa Nakamura, Yasuaki Shimoishi, Izumi C. Mori, Yoshiyuki Murata

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Abstract

We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca2+ oscillation in ABA signal pathway of Arabidopsis guard cells.

Original languageEnglish
Pages (from-to)2048-2055
Number of pages8
JournalJournal of Plant Physiology
Volume168
Issue number17
DOIs
Publication statusPublished - Nov 15 2011

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Keywords

  • Abscisic acid
  • Cytosolic calcium
  • Glutathione
  • Reactive oxygen species
  • Stomatal closure

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

Cite this

Okuma, E., Jahan, M. S., Munemasa, S., Hossain, M. A., Muroyama, D., Islam, M. M., Ogawa, K., Watanabe-Sugimoto, M., Nakamura, Y., Shimoishi, Y., Mori, I. C., & Murata, Y. (2011). Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis. Journal of Plant Physiology, 168(17), 2048-2055. https://doi.org/10.1016/j.jplph.2011.06.002