Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis

Eiji Okuma, Md Sarwar Jahan, Shintaro Munemasa, Mohammad Anowar Hossain, Daichi Muroyama, Mohammad Mahbub Islam, Ken'ichi Ogawa, Megumi Watanabe-Sugimoto, Yoshimasa Nakamura, Yasuaki Shimoishi, Izumi Mori, Yoshiyuki Murata

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca2+ oscillation in ABA signal pathway of Arabidopsis guard cells.

Original languageEnglish
Pages (from-to)2048-2055
Number of pages8
JournalJournal of Plant Physiology
Volume168
Issue number17
DOIs
Publication statusPublished - Nov 15 2011

Fingerprint

Abscisic Acid
Arabidopsis
Glutathione
abscisic acid
glutathione
guard cells
Dinitrochlorobenzene
calcium
oscillation
Glutamate-Cysteine Ligase
mutants
ligases
signal transduction
reactive oxygen species
Reactive Oxygen Species
Signal Transduction
plasma membrane
esters
Cell Membrane
biosynthesis

Keywords

  • Abscisic acid
  • Cytosolic calcium
  • Glutathione
  • Reactive oxygen species
  • Stomatal closure

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Agronomy and Crop Science

Cite this

Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis. / Okuma, Eiji; Jahan, Md Sarwar; Munemasa, Shintaro; Hossain, Mohammad Anowar; Muroyama, Daichi; Islam, Mohammad Mahbub; Ogawa, Ken'ichi; Watanabe-Sugimoto, Megumi; Nakamura, Yoshimasa; Shimoishi, Yasuaki; Mori, Izumi; Murata, Yoshiyuki.

In: Journal of Plant Physiology, Vol. 168, No. 17, 15.11.2011, p. 2048-2055.

Research output: Contribution to journalArticle

Okuma, E, Jahan, MS, Munemasa, S, Hossain, MA, Muroyama, D, Islam, MM, Ogawa, K, Watanabe-Sugimoto, M, Nakamura, Y, Shimoishi, Y, Mori, I & Murata, Y 2011, 'Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis', Journal of Plant Physiology, vol. 168, no. 17, pp. 2048-2055. https://doi.org/10.1016/j.jplph.2011.06.002
Okuma, Eiji ; Jahan, Md Sarwar ; Munemasa, Shintaro ; Hossain, Mohammad Anowar ; Muroyama, Daichi ; Islam, Mohammad Mahbub ; Ogawa, Ken'ichi ; Watanabe-Sugimoto, Megumi ; Nakamura, Yoshimasa ; Shimoishi, Yasuaki ; Mori, Izumi ; Murata, Yoshiyuki. / Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis. In: Journal of Plant Physiology. 2011 ; Vol. 168, No. 17. pp. 2048-2055.
@article{73bfa11dfa84447b8e8c70f60a9bac34,
title = "Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis",
abstract = "We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca2+ oscillation in ABA signal pathway of Arabidopsis guard cells.",
keywords = "Abscisic acid, Cytosolic calcium, Glutathione, Reactive oxygen species, Stomatal closure",
author = "Eiji Okuma and Jahan, {Md Sarwar} and Shintaro Munemasa and Hossain, {Mohammad Anowar} and Daichi Muroyama and Islam, {Mohammad Mahbub} and Ken'ichi Ogawa and Megumi Watanabe-Sugimoto and Yoshimasa Nakamura and Yasuaki Shimoishi and Izumi Mori and Yoshiyuki Murata",
year = "2011",
month = "11",
day = "15",
doi = "10.1016/j.jplph.2011.06.002",
language = "English",
volume = "168",
pages = "2048--2055",
journal = "Journal of Plant Physiology",
issn = "0176-1617",
publisher = "Urban und Fischer Verlag GmbH und Co. KG",
number = "17",

}

TY - JOUR

T1 - Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis

AU - Okuma, Eiji

AU - Jahan, Md Sarwar

AU - Munemasa, Shintaro

AU - Hossain, Mohammad Anowar

AU - Muroyama, Daichi

AU - Islam, Mohammad Mahbub

AU - Ogawa, Ken'ichi

AU - Watanabe-Sugimoto, Megumi

AU - Nakamura, Yoshimasa

AU - Shimoishi, Yasuaki

AU - Mori, Izumi

AU - Murata, Yoshiyuki

PY - 2011/11/15

Y1 - 2011/11/15

N2 - We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca2+ oscillation in ABA signal pathway of Arabidopsis guard cells.

AB - We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca2+ oscillation in ABA signal pathway of Arabidopsis guard cells.

KW - Abscisic acid

KW - Cytosolic calcium

KW - Glutathione

KW - Reactive oxygen species

KW - Stomatal closure

UR - http://www.scopus.com/inward/record.url?scp=80053934169&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80053934169&partnerID=8YFLogxK

U2 - 10.1016/j.jplph.2011.06.002

DO - 10.1016/j.jplph.2011.06.002

M3 - Article

C2 - 21764168

AN - SCOPUS:80053934169

VL - 168

SP - 2048

EP - 2055

JO - Journal of Plant Physiology

JF - Journal of Plant Physiology

SN - 0176-1617

IS - 17

ER -