Negative regulation of abscisic acid-induced stomatal closure by glutathione in Arabidopsis

Eiji Okuma, Md Sarwar Jahan, Shintaro Munemasa, Mohammad Anowar Hossain, Daichi Muroyama, Mohammad Mahbub Islam, Ken'ichi Ogawa, Megumi Watanabe, Yoshimasa Nakamura, Yasuaki Shimoishi, Izumi C. Mori, Yoshiyuki Murata

Research output: Contribution to journalArticlepeer-review

57 Citations (Scopus)

Abstract

We found that glutathione (GSH) is involved in abscisic acid (ABA)-induced stomatal closure. Regulation of ABA signaling by GSH in guard cells was investigated using an Arabidopsis mutant, cad2-1, that is deficient in the first GSH biosynthesis enzyme, γ-glutamylcysteine synthetase, and a GSH-decreasing chemical, 1-chloro-2,4-dinitrobenzene (CDNB). Glutathione contents in guard cells decreased along with ABA-induced stomatal closure. Decreasing GSH by both the cad2-1 mutation and CDNB treatment enhanced ABA-induced stomatal closure. Glutathione monoethyl ester (GSHmee) restored the GSH level in cad2-1 guard cells and complemented the stomatal phenotype of the mutant. Depletion of GSH did not significantly increase ABA-induced production of reactive oxygen species in guard cells and GSH did not affect either activation of plasma membrane Ca2+-permeable channel currents by ABA or oscillation of the cytosolic free Ca2+ concentration induced by ABA. These results indicate that GSH negatively modulates a signal component other than ROS production and Ca2+ oscillation in ABA signal pathway of Arabidopsis guard cells.

Original languageEnglish
Pages (from-to)2048-2055
Number of pages8
JournalJournal of Plant Physiology
Volume168
Issue number17
DOIs
Publication statusPublished - Nov 15 2011

Keywords

  • Abscisic acid
  • Cytosolic calcium
  • Glutathione
  • Reactive oxygen species
  • Stomatal closure

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science
  • Plant Science

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