Negative correlation between the ratio of k+ to na+ and proline accumulation in tobacco suspension cells

Eiji Okuma, Mikiro Tada, Kenji Soeda, Miho Fukuda, Yoshiyuki Murata

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

The concentrations of K+, Na+, and proline and the ratio of K+ to Na+ (K+ / Na+) were analyzed in NaCl-unadapted and NaCl-adapted tobacco (Nicotiana tabacum) cells in suspension culture. At 3 to 5 d after inoculation, the NaCl-unadapted cells cultured in 100 mmol L−1 NaCl saline culture medium (Na100 medium) accumulated 28.7 mmol L−1 proline with a low ratio of K+ to Na+ (= 2.8) and the NaCl-adapted cells cultured in the Na100 medium contained 6.28 mmol L−1 proline with a high K+ / Na+ ratio (≧ 7.5). The contents of amino acids for the NaCl-adapted cells in the Na100 medium were similar to those for the NaCl-unadapted cells in a modified LS medium (standard medium). At 14 d after inoculation, the NaCl-unadapted cells in the Na100 medium contained 4.77 mmol L−1 proline and restored the K+ / Na+ ratio from 2.8 to 6.2. These results indicate the presence of a negative correlation between the K+ / Na+ ratio and proline accumulation and suggest that a balance between the K+ / Na+ ratio and proline accumulation may be the factor involved in determining the salt tolerance of plant cells.

Original languageEnglish
Pages (from-to)753-757
Number of pages5
JournalSoil Science and Plant Nutrition
Volume48
Issue number5
DOIs
Publication statusPublished - Oct 1 2002

Keywords

  • K / Na ratio
  • Nicotiana tabacum
  • Proline
  • Salt stress
  • Tobacco

ASJC Scopus subject areas

  • Soil Science
  • Plant Science

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