Negative correlation between the ratio of K+ to Na+ and proline accumulation in tobacco suspension cells

Eiji Okuma, Kenji Soeda, Miho Fukuda, Mikiro Tada, Yoshiyuki Murata

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The concentrations of K+, Na+, and proline and the ratio of K+ to Na+ (K+ / Na+) were analyzed in NaCl-unadapted and NaCl-adapted tobacco (Nicotiana tabacum) cells in suspension culture. At 3 to 5 d after inoculation, the NaCl-unadapted cells cultured in 100 mmol L-1 NaCl saline culture medium (Na100 medium) accumulated 28.7 mmol L-1 proline with a low ratio of K+ to Na+ (= 2.8) and the NaCl-adapted cells cultured in the Na100 medium contained 6.28 mmol L-1 proline with a high K+ / Na+ ratio (≥ 7.5). The contents of amino acids for the NaCl-adapted cells in the Na100 medium were similar to those for the NaCl-unadapted cells in a modified LS medium (standard medium). At 14 d after inoculation, the NaCl-unadapted cells in the Na100 medium contained 4.77 mmol L-1 proline and restored the K+ / Na+ ratio from 2.8 to 6.2. These results indicate the presence of a negative correlation between the K+ / Na+ ratio and proline accumulation and suggest that a balance between the K+ / Na+ ratio and proline accumulation may be the factor involved in determining the salt tolerance of plant cells.

Original languageEnglish
Pages (from-to)753-757
Number of pages5
JournalSoil Science and Plant Nutrition
Volume48
Issue number5
Publication statusPublished - Oct 2002

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tobacco
cell suspension culture
inoculation
proline
amino acid
tolerance
salt
cultured cells
cells
salt tolerance
Nicotiana tabacum
culture media
amino acids

Keywords

  • K / Na ratio
  • Nicotiana tabacum
  • Proline
  • Salt stress
  • Tobacco

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Soil Science

Cite this

Negative correlation between the ratio of K+ to Na+ and proline accumulation in tobacco suspension cells. / Okuma, Eiji; Soeda, Kenji; Fukuda, Miho; Tada, Mikiro; Murata, Yoshiyuki.

In: Soil Science and Plant Nutrition, Vol. 48, No. 5, 10.2002, p. 753-757.

Research output: Contribution to journalArticle

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AU - Soeda, Kenji

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AU - Murata, Yoshiyuki

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N2 - The concentrations of K+, Na+, and proline and the ratio of K+ to Na+ (K+ / Na+) were analyzed in NaCl-unadapted and NaCl-adapted tobacco (Nicotiana tabacum) cells in suspension culture. At 3 to 5 d after inoculation, the NaCl-unadapted cells cultured in 100 mmol L-1 NaCl saline culture medium (Na100 medium) accumulated 28.7 mmol L-1 proline with a low ratio of K+ to Na+ (= 2.8) and the NaCl-adapted cells cultured in the Na100 medium contained 6.28 mmol L-1 proline with a high K+ / Na+ ratio (≥ 7.5). The contents of amino acids for the NaCl-adapted cells in the Na100 medium were similar to those for the NaCl-unadapted cells in a modified LS medium (standard medium). At 14 d after inoculation, the NaCl-unadapted cells in the Na100 medium contained 4.77 mmol L-1 proline and restored the K+ / Na+ ratio from 2.8 to 6.2. These results indicate the presence of a negative correlation between the K+ / Na+ ratio and proline accumulation and suggest that a balance between the K+ / Na+ ratio and proline accumulation may be the factor involved in determining the salt tolerance of plant cells.

AB - The concentrations of K+, Na+, and proline and the ratio of K+ to Na+ (K+ / Na+) were analyzed in NaCl-unadapted and NaCl-adapted tobacco (Nicotiana tabacum) cells in suspension culture. At 3 to 5 d after inoculation, the NaCl-unadapted cells cultured in 100 mmol L-1 NaCl saline culture medium (Na100 medium) accumulated 28.7 mmol L-1 proline with a low ratio of K+ to Na+ (= 2.8) and the NaCl-adapted cells cultured in the Na100 medium contained 6.28 mmol L-1 proline with a high K+ / Na+ ratio (≥ 7.5). The contents of amino acids for the NaCl-adapted cells in the Na100 medium were similar to those for the NaCl-unadapted cells in a modified LS medium (standard medium). At 14 d after inoculation, the NaCl-unadapted cells in the Na100 medium contained 4.77 mmol L-1 proline and restored the K+ / Na+ ratio from 2.8 to 6.2. These results indicate the presence of a negative correlation between the K+ / Na+ ratio and proline accumulation and suggest that a balance between the K+ / Na+ ratio and proline accumulation may be the factor involved in determining the salt tolerance of plant cells.

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