Abstract
Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation; in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi.
| Original language | English |
|---|---|
| Pages (from-to) | 1669-1673 |
| Number of pages | 5 |
| Journal | Bioconjugate Chemistry |
| Volume | 24 |
| Issue number | 10 |
| DOIs | |
| Publication status | Published - Oct 16 2013 |
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ASJC Scopus subject areas
- Biotechnology
- Bioengineering
- Organic Chemistry
- Pharmaceutical Science
- Biomedical Engineering
- Pharmacology
Cite this
Near-infrared light-directed RNAi using a photosensitive carrier molecule. / Matsushita-Ishiodori, Yuka; Morinaga, Mika; Watanabe, Kazunori; Ohtsuki, Takashi.
In: Bioconjugate Chemistry, Vol. 24, No. 10, 16.10.2013, p. 1669-1673.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Near-infrared light-directed RNAi using a photosensitive carrier molecule
AU - Matsushita-Ishiodori, Yuka
AU - Morinaga, Mika
AU - Watanabe, Kazunori
AU - Ohtsuki, Takashi
PY - 2013/10/16
Y1 - 2013/10/16
N2 - Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation; in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi.
AB - Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation; in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi.
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UR - http://www.scopus.com/inward/citedby.url?scp=84886044163&partnerID=8YFLogxK
U2 - 10.1021/bc4001195
DO - 10.1021/bc4001195
M3 - Article
C2 - 23998791
AN - SCOPUS:84886044163
VL - 24
SP - 1669
EP - 1673
JO - Bioconjugate Chemistry
JF - Bioconjugate Chemistry
SN - 1043-1802
IS - 10
ER -