Near-infrared light-directed RNAi using a photosensitive carrier molecule

Yuka Matsushita-Ishiodori; Mika Morinaga; Kazunori Watanabe; Takashi Ohtsuki

doi:10.1021/bc4001195

Near-infrared light-directed RNAi using a photosensitive carrier molecule

Yuka Matsushita-Ishiodori, Mika Morinaga, Kazunori Watanabe, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

11 Citations (Scopus)

Abstract

Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation; in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi.

Original language	English
Pages (from-to)	1669-1673
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	24
Issue number	10
DOIs	https://doi.org/10.1021/bc4001195
Publication status	Published - Oct 16 2013

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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10.1021/bc4001195

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Near-infrared light-directed RNAi using a photosensitive carrier molecule

Abstract

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