Near-infrared light-directed RNAi using a photosensitive carrier molecule

Yuka Matsushita-Ishiodori; Mika Morinaga; Kazunori Watanabe; Takashi Ohtsuki

doi:10.1021/bc4001195

Near-infrared light-directed RNAi using a photosensitive carrier molecule

Yuka Matsushita-Ishiodori, Mika Morinaga, Kazunori Watanabe, Takashi Ohtsuki

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

Abstract

Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation; in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi.

Original language	English
Pages (from-to)	1669-1673
Number of pages	5
Journal	Bioconjugate Chemistry
Volume	24
Issue number	10
DOIs	https://doi.org/10.1021/bc4001195
Publication status	Published - Oct 16 2013

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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