Near-infrared light-directed RNAi using a photosensitive carrier molecule

Yuka Matsushita-Ishiodori, Mika Morinaga, Kazunori Watanabe, Takashi Ohtsuki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Controlled activation of small RNAs, such as small interfering RNA, in cells is very useful for various biological applications. Light is an effective inducer of controlled activation; in particular, near-infrared light is favorable because it can penetrate deeper into tissues than UV or visible light. In this study, near-infrared light control of RNA interference (RNAi) was demonstrated in mammalian cells using a photosensitive RNA carrier molecule, consisting of an RNA carrier protein and a fluorochrome. The photosensitive carrier molecule was identified from six candidates, each with a different fluorochrome. Using this carrier molecule, cytosolic RNA delivery and RNAi can be triggered by near-infrared light. Cytotoxicity was not observed after photoinduction of RNAi.

Original languageEnglish
Pages (from-to)1669-1673
Number of pages5
JournalBioconjugate Chemistry
Volume24
Issue number10
DOIs
Publication statusPublished - Oct 16 2013

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RNA Interference
RNA
Infrared radiation
Light
Molecules
Fluorescent Dyes
Chemical activation
Ultraviolet Rays
Small Interfering RNA
Carrier Proteins
Cytotoxicity
Cells
Tissue

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Organic Chemistry
  • Pharmaceutical Science
  • Biomedical Engineering
  • Pharmacology

Cite this

Near-infrared light-directed RNAi using a photosensitive carrier molecule. / Matsushita-Ishiodori, Yuka; Morinaga, Mika; Watanabe, Kazunori; Ohtsuki, Takashi.

In: Bioconjugate Chemistry, Vol. 24, No. 10, 16.10.2013, p. 1669-1673.

Research output: Contribution to journalArticle

Matsushita-Ishiodori, Yuka ; Morinaga, Mika ; Watanabe, Kazunori ; Ohtsuki, Takashi. / Near-infrared light-directed RNAi using a photosensitive carrier molecule. In: Bioconjugate Chemistry. 2013 ; Vol. 24, No. 10. pp. 1669-1673.
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