Naturally occurring lung CD4+CD25+ T cell regulation of airway allergic responses depends on IL-10 induction of TGF- β1

Anthony Joetham, Katsuyuki Takada, Christian Taube, Nobuaki Miyahara, Satoko Matsubara, Toshiyuki Koya, Yeong Ho Rha, Azzeddine Dakhama, Erwin W. Gelfand

Research output: Contribution to journalArticle

136 Citations (Scopus)

Abstract

Peripheral tolerance to allergens is mediated in large part by the naturally occurring lung CD4+CD25+ T cells, but their effects on allergen-induced airway responsiveness have not been well defined. Intratracheal, but not i.v., administration of naive lung CD4 +CD25+ T cells before allergen challenge of sensitized mice, similar to the administration of the combination of rIL-10 and rTGF-β, resulted in reduced airway hyperresponsiveness (AHR) and inflammation, lower levels of Th2 cytokines, higher levels of IL-10 and TGF-β, and less severe lung histopathology. Significantly, CD4 +CD25+ T cells isolated from IL-10-/- mice had no effect on AHR and inflammation, but when incubated with rIL-10 before transfer, suppressed AHR, and inflammation, and was associated with elevated levels of bronchoalveolar lavage TGF-β levels. By analogy, anti-TGF-β treatment reduced regulatory T cell activity. These data identify naturally occurring lung CD4+CD25+ T cells as capable of regulating lung allergic responses in an IL-10- and TGF-β-dependent manner.

Original languageEnglish
Pages (from-to)1433-1442
Number of pages10
JournalJournal of Immunology
Volume178
Issue number3
Publication statusPublished - Feb 1 2007
Externally publishedYes

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Interleukin-10
T-Lymphocytes
Lung
Allergens
Inflammation
Peripheral Tolerance
Bronchoalveolar Lavage
Regulatory T-Lymphocytes
Cytokines
Therapeutics

ASJC Scopus subject areas

  • Immunology

Cite this

Joetham, A., Takada, K., Taube, C., Miyahara, N., Matsubara, S., Koya, T., ... Gelfand, E. W. (2007). Naturally occurring lung CD4+CD25+ T cell regulation of airway allergic responses depends on IL-10 induction of TGF- β1 . Journal of Immunology, 178(3), 1433-1442.

Naturally occurring lung CD4+CD25+ T cell regulation of airway allergic responses depends on IL-10 induction of TGF- β1 . / Joetham, Anthony; Takada, Katsuyuki; Taube, Christian; Miyahara, Nobuaki; Matsubara, Satoko; Koya, Toshiyuki; Rha, Yeong Ho; Dakhama, Azzeddine; Gelfand, Erwin W.

In: Journal of Immunology, Vol. 178, No. 3, 01.02.2007, p. 1433-1442.

Research output: Contribution to journalArticle

Joetham, A, Takada, K, Taube, C, Miyahara, N, Matsubara, S, Koya, T, Rha, YH, Dakhama, A & Gelfand, EW 2007, 'Naturally occurring lung CD4+CD25+ T cell regulation of airway allergic responses depends on IL-10 induction of TGF- β1 ', Journal of Immunology, vol. 178, no. 3, pp. 1433-1442.
Joetham, Anthony ; Takada, Katsuyuki ; Taube, Christian ; Miyahara, Nobuaki ; Matsubara, Satoko ; Koya, Toshiyuki ; Rha, Yeong Ho ; Dakhama, Azzeddine ; Gelfand, Erwin W. / Naturally occurring lung CD4+CD25+ T cell regulation of airway allergic responses depends on IL-10 induction of TGF- β1 . In: Journal of Immunology. 2007 ; Vol. 178, No. 3. pp. 1433-1442.
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