Natural selection on PHYE by latitude in the Japanese archipelago

Insight from locus specific phylogeographic structure in Arcterica nana (Ericaceae)

Hajime Ikeda, Hiroaki Setoguchi

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Phytochromes play a key role in allowing plants to monitor their surrounding environment and, conversely, adaptation to local environments has driven the evolutionary history of phytochromes. As a result of natural selection, polymorphisms in phytochrome genes would thus be expected to exhibit locus-specific phylogeographic structure. To evaluate this hypothesis, we conducted a phylogeographic investigation based on four nuclear genes, including two phytochrome genes (PHYB and PHYE) using 155 samples of Arcterica nana from the entire range of the Japanese archipelago. Bayesian clustering revealed geographic differentiation between northern and southern Japan when all four genes were included. However, this geographic differentiation is inconsistent with previously reported genetic structure of genome-wide polymorphisms based on amplified fragment length polymorphisms, as these did not show geographic differentiation throughout the Japanese archipelago. In contrast, the north-south differentiation was not apparent when PHYE was excluded. This indicates that PHYE alone could be responsible for the north-south differentiation (FCT = 0.15, P <0.001). Furthermore, a single nonsynonymous polymorphism (C360T) strongly contributed to geographic differentiation (FCT = 0.57, P <0.001) and its corresponding amino acid replacement (P120L) was significantly under positive selection based on maximum likelihood analysis (P = 0.98). Consequently, the locus-specific geographic differentiation in PHYE could be caused by natural selection, suggesting the involvement of PHYE in local adaptation between populations of A. nana in northern and southern Japan. This finding is consistent with a previous study on Cardamine nipponica, indicating the importance of PHYE for local adaptation in Japanese alpine plants.

Original languageEnglish
Pages (from-to)2779-2791
Number of pages13
JournalMolecular Ecology
Volume19
Issue number13
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

Fingerprint

Ericaceae
Phytochrome
Genetic Selection
phytochrome
natural selection
archipelago
loci
genetic polymorphism
polymorphism
Genes
Cardamine
Japan
genes
gene
local adaptation
alpine plants
probability analysis
Genetic Structures
amplified fragment length polymorphism
Cluster Analysis

Keywords

  • Japanese archipelago
  • latitudinal cline
  • local adaptation
  • phylogeography
  • phytochrome

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics
  • Medicine(all)

Cite this

Natural selection on PHYE by latitude in the Japanese archipelago : Insight from locus specific phylogeographic structure in Arcterica nana (Ericaceae). / Ikeda, Hajime; Setoguchi, Hiroaki.

In: Molecular Ecology, Vol. 19, No. 13, 07.2010, p. 2779-2791.

Research output: Contribution to journalArticle

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