Abstract
Everted membrane vesicles were prepared from cells of Pseudomonas aeruginosa, and cation/H+ antiport was measured. We observed activities of Na+/H+ antiport, Li+/H+ antiport and K+/H+ antiport. Judging from the competition pattern, it seems that there are at least two types of antiporter, a Na+(Li+)/H+ antiporter and a K+/H+ antiporter. Na+ was a good substrate for the Na+ (Li+)/H+ system, whereas Li+ was a poor substrate. Although the K(m) value for Na+ (or Li+) was similar to those in Escherichia coli Na+/H+ antiporters, the V(max) value for Na+ (or Li+) was much smaller in the P. aeruginosa antiporter than in the E. coli antiporters. Growth of P. aeruginosa was strongly inhibited by 0.4 M LiCl, but not by NaCl or KCl.
Original language | English |
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Pages (from-to) | 621-624 |
Number of pages | 4 |
Journal | Biological and Pharmaceutical Bulletin |
Volume | 20 |
Issue number | 6 |
DOIs | |
Publication status | Published - Jun 1997 |
Keywords
- Amiloride
- Antiporter
- Lithium
- P. aeruginosa
- Sodium
ASJC Scopus subject areas
- Pharmacology
- Pharmaceutical Science