Nanolayering of phosphoric acid ester monomer on enamel and dentin

Kumiko Yoshihara, Yasuhiro Yoshida, Satoshi Hayakawa, Noriyuki Nagaoka, Masao Irie, Tatsuyuki Ogawa, Kirsten L. Van Landuyt, Akiyoshi Osaka, Kazuomi Suzuki, Shougo Minagi, Bart Van Meerbeek

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Following the "adhesion-decalcification" concept, specific functional monomers possess the capacity to primary chemically interact with hydroxyapatite (HAp). Such ionic bonding with synthetic HAp has been demonstrated for 10-methacryloyloxydecyl dihydrogen phosphate (10-MDP), manifest as self-assembled "nanolayering". In continuation of that basic research this study aimed to explore whether nanolayering also occurs on enamel and dentin when a 10-MDP primer is applied following a common clinical application protocol. Therefore, the interaction of an experimental 10-MDP primer and a control, commercially available, 10-MDP-based primer (Clearfil SE Bond primer (C-SE), Kuraray) with enamel and dentin was characterized by X-ray diffraction (XRD), complemented with transmission electron microscopy interfacial ultrastructural data upon their reaction with enamel and dentin. In addition, XRD was used to study the effect of the concentration of 10-MDP on nanolayering on dentin. Finally, the stability of the nanolayers was determined by measuring the bond strength to enamel and dentin when a photoinitiator was added to the experimental primer or when interfacial polymerization depended solely on the photoinitiator supplied with the subsequently applied adhesive resin. XRD confirmed nanolayering on enamel and dentin, which was significantly greater on dentin than on enamel, and also when the surface was actively rubbed with the primer. Nanolayering was also proportional to the concentration of 10-MDP in the primer. Finally, the experimental primer needed the photoinitiator to obtain a tensile bond strength to dentin comparable with that of the control C-SE primer (which also contains a photoinitiator), but not when bonded to enamel. It is concluded that self-assembled nanolayering occurs on enamel and dentin, even when following a clinically used application protocol. The lower bonding effectiveness of mild self-etch adhesives to enamel should be ascribed in part to a lower chemical reactivity (nanolayering) with enamel HAp.

Original languageEnglish
Pages (from-to)3187-3195
Number of pages9
JournalActa Biomaterialia
Volume7
Issue number8
DOIs
Publication statusPublished - Aug 2011

Fingerprint

Organophosphates
Enamels
Phosphoric acid
Dentin
Dental Enamel
Esters
Monomers
Phosphates
Durapatite
Hydroxyapatite
X-Ray Diffraction
X ray diffraction
Adhesives
Chemical reactivity
Tensile Strength
Clinical Protocols
Transmission Electron Microscopy
Polymerization
methacryloyloxydecyl dihydrogen phosphate
Adhesion

Keywords

  • Adhesive
  • Dentin
  • Enamel
  • Functional monomer
  • X-ray diffraction

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Biotechnology
  • Biochemistry
  • Molecular Biology

Cite this

Nanolayering of phosphoric acid ester monomer on enamel and dentin. / Yoshihara, Kumiko; Yoshida, Yasuhiro; Hayakawa, Satoshi; Nagaoka, Noriyuki; Irie, Masao; Ogawa, Tatsuyuki; Van Landuyt, Kirsten L.; Osaka, Akiyoshi; Suzuki, Kazuomi; Minagi, Shougo; Van Meerbeek, Bart.

In: Acta Biomaterialia, Vol. 7, No. 8, 08.2011, p. 3187-3195.

Research output: Contribution to journalArticle

Yoshihara, K, Yoshida, Y, Hayakawa, S, Nagaoka, N, Irie, M, Ogawa, T, Van Landuyt, KL, Osaka, A, Suzuki, K, Minagi, S & Van Meerbeek, B 2011, 'Nanolayering of phosphoric acid ester monomer on enamel and dentin', Acta Biomaterialia, vol. 7, no. 8, pp. 3187-3195. https://doi.org/10.1016/j.actbio.2011.04.026
Yoshihara, Kumiko ; Yoshida, Yasuhiro ; Hayakawa, Satoshi ; Nagaoka, Noriyuki ; Irie, Masao ; Ogawa, Tatsuyuki ; Van Landuyt, Kirsten L. ; Osaka, Akiyoshi ; Suzuki, Kazuomi ; Minagi, Shougo ; Van Meerbeek, Bart. / Nanolayering of phosphoric acid ester monomer on enamel and dentin. In: Acta Biomaterialia. 2011 ; Vol. 7, No. 8. pp. 3187-3195.
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