NaJAZh regulates a subset of defense responses against herbivores and spontaneous leaf necrosis in Nicotiana attenuata plants

Youngjoo Oh, Ian T. Baldwin, Ivan Galis

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most JAZ genes showed strong expression in the elicited leaves, but NaJAZg was mainly expressed in roots. Another novel herbivory-elicited gene, NaJAZh, was analyzed in detail. RNA interference suppression of this gene in inverted-repeat (ir)JAZh plants deregulated a specific branch of jasmonic acid-dependent direct and indirect defenses: irJAZh plants showed greater trypsin protease inhibitor activity, 17-hydroxygeranyllinalool diterpene glycosides accumulation, and emission of volatile organic compounds from leaves. Silencing of NaJAZh also revealed a novel cross talk in JAZ-regulated secondary metabolism, as irJAZh plants had significantly reduced nicotine levels. In addition, irJAZh spontaneously developed leaf necrosis during the transition to flowering. Because the lesions closely correlated with the elevated expression of programmed cell death genes and the accumulations of salicylic acid and hydrogen peroxide in the leaves, we propose a novel role of the NaJAZh protein as a repressor of necrosis and/or programmed cell death during plant development.

Original languageEnglish
Pages (from-to)769-788
Number of pages20
JournalPlant Physiology
Volume159
Issue number2
DOIs
Publication statusPublished - Jun 2012

Fingerprint

Herbivory
Tobacco
necrosis
Necrosis
herbivores
Genes
leaves
jasmonic acid
genes
Cell Death
tobacco
apoptosis
Secondary Metabolism
Volatile Organic Compounds
lesions (plant)
Plant Leaves
Plant Development
Trypsin Inhibitors
Salicylic Acid
Diterpenes

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Physiology

Cite this

NaJAZh regulates a subset of defense responses against herbivores and spontaneous leaf necrosis in Nicotiana attenuata plants. / Oh, Youngjoo; Baldwin, Ian T.; Galis, Ivan.

In: Plant Physiology, Vol. 159, No. 2, 06.2012, p. 769-788.

Research output: Contribution to journalArticle

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