NAI1 gene encodes a basic-helix-loop-helix-type putative transcription factor that regulates the formation of an endoplasmic reticulum-derived structure, the ER body

Ryo Matsushima, Yoichiro Fukao, Mikio Nishimura, Ikuko Hara-Nishimura

Research output: Contribution to journalArticle

65 Citations (Scopus)

Abstract

Plant cells develop various types of endoplasmic reticulum (ER)-derived structures with specific functions. ER body, an ER-derived compartment in Arabidopsis thaliana, is a spindle-shaped structure. The NAI1 gene regulates the development of ER bodies because mutation of NAI1 abolishes the formation of ER bodies. To better understand the role of NAI1, we cloned the NAI1 gene using a positional cloning strategy. The nai1-1 mutant had a single nucleotide change at an intron acceptor site of At2g22770 (NAI1 gene). Because of this mutation, aberrant splicing of NAI1 mRNA occurs in the nai1-1 mutant. NAI1 encodes a transcription factor that has a basic-helix-loop-helix (bHLH) domain. Transient expression of NAI1 induced ER bodies in the nsi1-1 mutant. Two-dimensional electrophoresis and RT-PCR analyses showed that a putative lectin was depressed at both the mRNA and protein levels in nai1 mutants, as was a β-glucosidase (PYK10). Our results provide direct evidence that a bHLH protein plays a role in the formation of ER bodies.

Original languageEnglish
Pages (from-to)1536-1549
Number of pages14
JournalPlant Cell
Volume16
Issue number6
DOIs
Publication statusPublished - Jun 2004
Externally publishedYes

Fingerprint

Basic Helix-Loop-Helix Transcription Factors
Endoplasmic Reticulum
endoplasmic reticulum
Transcription Factors
transcription factors
Genes
Glucosidases
Messenger RNA
genes
Cloning
mutants
Electrophoresis
Lectins
Introns
Proteins
Nucleotides
Helix-Loop-Helix Motifs
basic helix-loop-helix transcription factors
mutation
Mutation

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

NAI1 gene encodes a basic-helix-loop-helix-type putative transcription factor that regulates the formation of an endoplasmic reticulum-derived structure, the ER body. / Matsushima, Ryo; Fukao, Yoichiro; Nishimura, Mikio; Hara-Nishimura, Ikuko.

In: Plant Cell, Vol. 16, No. 6, 06.2004, p. 1536-1549.

Research output: Contribution to journalArticle

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