NADH-dependent glutamate synthase participated in ammonium assimilation in Arabidopsis root

Soichi Kojima, Noriyuki Konishi, Marcel Pascal Beier, Keiki Ishiyama, Ikumi Maru, Toshihiko Hayakawa, Tomoyuki Yamaya

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9 Citations (Scopus)

Abstract

Higher plants have 2 GOGAT species, Fd-GOGAT and NADHGOGAT. While Fd-GOGAT mainly assimilates ammonium in leaves, which is derived from photorespiration, the function of NADH-GOGAT, which is highly expressed in roots,1 needs to be elucidated. The aim of this study was to clarify the role of NADH-GOGAT in Arabidopsis roots. The supply of ammonium to the roots caused an accumulation of NADH-GOGAT, while Fd-GOGAT 1 and Fd-GOGAT 2 showed no response. A promoter–GUS fusion analysis and immunohistochemistry showed that NADH-GOGAT was located in nongreen tissues like vascular bundles, shoot apical meristem, pollen, stigma, and roots. The localization of NADH-GOGAT and Fd-GOGAT was not overlapped. NADHGOGAT T-DNA insertion lines showed a reduction of glutamate and biomass under normal CO2 conditions. These data emphasizes the importance of NADHGOGAT in the ammonium assimilation of Arabidopsis roots.

Original languageEnglish
Article numbere29402
JournalPlant Signaling and Behavior
Volume9
Issue number8
DOIs
Publication statusPublished - Jun 4 2014
Externally publishedYes

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Keywords

  • Amino acids
  • Ammonium
  • Arabidopsis
  • Assimilation
  • CO
  • Glutamate
  • NADH-GOGAT
  • Root

ASJC Scopus subject areas

  • Plant Science

Cite this

Kojima, S., Konishi, N., Beier, M. P., Ishiyama, K., Maru, I., Hayakawa, T., & Yamaya, T. (2014). NADH-dependent glutamate synthase participated in ammonium assimilation in Arabidopsis root. Plant Signaling and Behavior, 9(8), [e29402]. https://doi.org/10.4161/psb.29402