Na+ (Li+)/H+ antiporter in Pseudomonas aeruginosa and effect of Li+ on cell growth

Kei Inaba, Junko Utsugi, Teruo Kuroda, Masaaki Tsuda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Everted membrane vesicles were prepared from cells of Pseudomonas aeruginosa, and cation/H+ antiport was measured. We observed activities of Na+/H+ antiport, Li+/H+ antiport and K+/H+ antiport. Judging from the competition pattern, it seems that there are at least two types of antiporter, a Na+(Li+)/H+ antiporter and a K+/H+ antiporter. Na+ was a good substrate for the Na+ (Li+)/H+ system, whereas Li+ was a poor substrate. Although the K(m) value for Na+ (or Li+) was similar to those in Escherichia coli Na+/H+ antiporters, the V(max) value for Na+ (or Li+) was much smaller in the P. aeruginosa antiporter than in the E. coli antiporters. Growth of P. aeruginosa was strongly inhibited by 0.4 M LiCl, but not by NaCl or KCl.

Original languageEnglish
Pages (from-to)621-624
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume20
Issue number6
Publication statusPublished - Jun 1997

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Antiporters
Sodium-Hydrogen Antiporter
Ion Transport
Pseudomonas aeruginosa
Growth
Escherichia coli
Cations
Membranes
sodium-lithium countertransporter

Keywords

  • Amiloride
  • Antiporter
  • Lithium
  • P. aeruginosa
  • Sodium

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Inaba, K., Utsugi, J., Kuroda, T., Tsuda, M., & Tsuchiya, T. (1997). Na+ (Li+)/H+ antiporter in Pseudomonas aeruginosa and effect of Li+ on cell growth. Biological and Pharmaceutical Bulletin, 20(6), 621-624.

Na+ (Li+)/H+ antiporter in Pseudomonas aeruginosa and effect of Li+ on cell growth. / Inaba, Kei; Utsugi, Junko; Kuroda, Teruo; Tsuda, Masaaki; Tsuchiya, Tomofusa.

In: Biological and Pharmaceutical Bulletin, Vol. 20, No. 6, 06.1997, p. 621-624.

Research output: Contribution to journalArticle

Inaba, K, Utsugi, J, Kuroda, T, Tsuda, M & Tsuchiya, T 1997, 'Na+ (Li+)/H+ antiporter in Pseudomonas aeruginosa and effect of Li+ on cell growth', Biological and Pharmaceutical Bulletin, vol. 20, no. 6, pp. 621-624.
Inaba, Kei ; Utsugi, Junko ; Kuroda, Teruo ; Tsuda, Masaaki ; Tsuchiya, Tomofusa. / Na+ (Li+)/H+ antiporter in Pseudomonas aeruginosa and effect of Li+ on cell growth. In: Biological and Pharmaceutical Bulletin. 1997 ; Vol. 20, No. 6. pp. 621-624.
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