N-linked sugar chain of 55-kDa royal jelly glycoprotein

Yoshinobu Kimura, Shin Ichiro Kajiyama, Jun Kanaeda, Tomomi Izukawa, Masami Yonekura

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

An N-linked sugar chain from 55-kDa royal jelly glycoprotein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507-509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz 1H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1 → 2Manα1 → 6(Manα1 → 2Manα1 → 3)Manα1 → 6(Manα1 → 2Manα1 → 2Manα1 → 3)Manβ1 → 4GlcNAcβ1 → 4GlcNAc, which is a non-processed high mannose type structure.

Original languageEnglish
Pages (from-to)2099-2102
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number12
Publication statusPublished - Dec 1996

Fingerprint

royal jelly
Sugars
glycoproteins
Glycoproteins
sugars
Mannosidases
Matrix-Assisted Laser Desorption-Ionization Mass Spectrometry
mannosidases
Acetylation
Mannose
Digestion
Cultured Cells
acetylation
mannose
Carbohydrates
Structural analysis
Liver
cultured cells
Rats
digestion

Keywords

  • insect glycoprotein
  • MALDI-TOF MS
  • N-glycan
  • PA-sugar chain
  • royal jelly

ASJC Scopus subject areas

  • Food Science
  • Applied Microbiology and Biotechnology
  • Chemistry (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology

Cite this

Kimura, Y., Kajiyama, S. I., Kanaeda, J., Izukawa, T., & Yonekura, M. (1996). N-linked sugar chain of 55-kDa royal jelly glycoprotein. Bioscience, Biotechnology and Biochemistry, 60(12), 2099-2102.

N-linked sugar chain of 55-kDa royal jelly glycoprotein. / Kimura, Yoshinobu; Kajiyama, Shin Ichiro; Kanaeda, Jun; Izukawa, Tomomi; Yonekura, Masami.

In: Bioscience, Biotechnology and Biochemistry, Vol. 60, No. 12, 12.1996, p. 2099-2102.

Research output: Contribution to journalArticle

Kimura, Y, Kajiyama, SI, Kanaeda, J, Izukawa, T & Yonekura, M 1996, 'N-linked sugar chain of 55-kDa royal jelly glycoprotein', Bioscience, Biotechnology and Biochemistry, vol. 60, no. 12, pp. 2099-2102.
Kimura Y, Kajiyama SI, Kanaeda J, Izukawa T, Yonekura M. N-linked sugar chain of 55-kDa royal jelly glycoprotein. Bioscience, Biotechnology and Biochemistry. 1996 Dec;60(12):2099-2102.
Kimura, Yoshinobu ; Kajiyama, Shin Ichiro ; Kanaeda, Jun ; Izukawa, Tomomi ; Yonekura, Masami. / N-linked sugar chain of 55-kDa royal jelly glycoprotein. In: Bioscience, Biotechnology and Biochemistry. 1996 ; Vol. 60, No. 12. pp. 2099-2102.
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