N-Linked sugar chain of 55-kda royal jelly glycoprotein

Yoshinobu Kimura, Shin Ichiro Kajiyama, Jun Kanaeda, Tomomi Izukawa, Masami Yonekura

Research output: Contribution to journalArticlepeer-review

33 Citations (Scopus)

Abstract

An N-linked sugar chain from 55-kDa royal jelly glycoportein (RJGP), which maintains the high viability of rat liver primary cultured cell and is a different molecular species from 350-kDa RJGP [Kimura et al., Biosci. Biotech. Biochem., 59, 507–509 (1995)], has been identified. The sugar chains were released by hydrazinolysis followed by N-acetylation and pyridylamination. The structural analysis of the pyridylaminated sugar chain was done by a combination of sequential exo-mannosidase digestions, MALDI-TOF MS, and 500 MHz 1H-NMR. For the carbohydrate moiety of 55-kDa RJGP, only one N-linked sugar chain has been detected. The structure has been found to be Manα1→2Manα1→6(Manα1→2Manα1→3)Manαl→6(Manα1→2Manα1→2Manα1 →3)Manβ1→4GlcNAcβl→4GlcNAc, which is a non-processed high mannose type structure.

Original languageEnglish
Pages (from-to)2099-2102
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume60
Issue number12
DOIs
Publication statusPublished - Jan 1996

Keywords

  • Insect glycoprotein
  • MALDI-TOF MS
  • N-glycan;
  • PA-sugar chain
  • Royal jelly

ASJC Scopus subject areas

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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