N-Linked Oligosaccharides of Glycoproteins from Ginkgo biloba Pollen, An Allergenic Pollen

Yoshinobu Kimura, Masashi Suzuki, Mariko Kimura

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

The pollen of Ginkgo biloba is one of the allergens that cause pollen allergy symptoms. The plant complex type N-glycans bearing β1-2 xylose and/or α1-3 fucose residue(s) linked to glycoallergens have been considered to be critical epitopes in various immune reactions. In this report, the structures of N-glycans of total glycoproteins prepared from Ginkgo biloba pollens were analyzed to confirm whether such plant complex type N-glycans occur in the pollen glycoproteins. The glycoproteins were extracted by SDS-Tris buffer. N-Glycans liberated from the pollen glycoprotein mixture by hydrazinolysis were labeled with 2-aminopyridine and the resulting pyridylaminated (PA-) N-glycans were purified by a combination of size-fractionation HPLC and reversed-phase HPLC. The structures of the PA-sugar chains were analyzed by a combination of two-dimensional sugar chain mapping, IS-MS, and MS/MS. The plant complex type structures (GlcNAc2Man3Xyl1 Fuc1GlcNAc2 (31%), GlcNAc2Man3Xyl1GlcNAc2 (5%), Man3Xyl1Fuc1GlcNAc2 (13%), GlcNAc1Man3 Xyl1Fuc1GlcNAc2 (8%), and GlcNAc1Man3Xyl1 GlcNAc2 (17%)) have been found among the N-glycans of the glycoproteins of Ginkgo biloba pollen, which might be candidates for the epitopes involved in Ginkgo pollen allergy. The remaining 26% of the total pollen N-glycans have the typical high-mannose type structures: Man8GlcNAc2 (11%) and Man6GlcNAc2 (15%).

Original languageEnglish
Pages (from-to)2001-2006
Number of pages6
JournalBioscience, Biotechnology and Biochemistry
Volume65
Issue number9
DOIs
Publication statusPublished - Sep 2001

Fingerprint

Ginkgo biloba
Oligosaccharides
Glycoproteins
Pollen
oligosaccharides
Polysaccharides
glycoproteins
polysaccharides
pollen
Allergies
Epitopes
hay fever
Sugars
Seasonal Allergic Rhinitis
Bearings (structural)
Antigen-antibody reactions
Allergens
epitopes
Xylose
High Pressure Liquid Chromatography

Keywords

  • Antigenic N-glycan
  • Ginkgo biloba
  • Plant glycoprotein
  • Pollen allergy
  • Sugar chains

ASJC Scopus subject areas

  • Food Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biotechnology
  • Chemistry (miscellaneous)
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

N-Linked Oligosaccharides of Glycoproteins from Ginkgo biloba Pollen, An Allergenic Pollen. / Kimura, Yoshinobu; Suzuki, Masashi; Kimura, Mariko.

In: Bioscience, Biotechnology and Biochemistry, Vol. 65, No. 9, 09.2001, p. 2001-2006.

Research output: Contribution to journalArticle

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