N-glycan metabolism and plant cell differentiation and growth

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Free N-glycans are present at micromolar concentrations in plant cells during their differentiation, growth and maturation stages, and might play a role in processes such as seed germination and fruit ripening. The structure of free N-glycans, which are found in hypocotyls and developing seeds and fruit, can be classified into two types: a high-mannose type (HMT) and a plant complex type (CT); the former, in most cases, has only one GlcNAc residue, while the latter has a chitobiose unit. It is thought that the enzyme endo-β-N- acetylglucosaminidase (endo-β-GlcNAc-ase) is involved in the production of HMT sugar chains, whereas the enzyme peptide:N-glycanase (PNGase) is involved in the production of plant CT sugar chains. However, the mechanism and significance of free N-glycan production in plant cells remain obscure. To characterize N-glycan metabolism and the physiological function of free sugar chains, we have investigated the substrate specificities, intracellular distributions, and gene structures of endo-β-GlcNAc-ase, PNGase, and α-mannosidase in various plants. Here, we report our discovery that endo-β-GlcNAc-ase activity begins to increase at a specific stage of tomato ripening, and that the amount of free N-glycans dramatically increases in conjunction with this event. In addition, the structural properties of free N-glycans also change notably as the fruit ripens. This review describes N-glycan metabolism in plant cells, and proposes a role for free sugar chains in the differentiation and growth of plants. The recent finding that plant CT sugar chains are immunoactive is also discussed.

Original languageEnglish
Pages (from-to)204-214
Number of pages11
JournalTrends in Glycoscience and Glycotechnology
Volume17
Issue number97
Publication statusPublished - Sep 2005

Fingerprint

Plant Cells
Metabolism
Polysaccharides
Cell Differentiation
Sugars
Growth
Fruits
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Fruit
Mannose
Seed
Seeds
Mannosidases
Hypocotyl
Acetylglucosaminidase
Enzymes
Lycopersicon esculentum
Germination
Substrate Specificity
Structural properties

Keywords

  • Deglycosylation enzyme
  • Free N-glycan
  • Immunological activity
  • N-glycan metabolism
  • Plant glycomics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

N-glycan metabolism and plant cell differentiation and growth. / Maeda, Megumi; Kimura, Yoshinobu.

In: Trends in Glycoscience and Glycotechnology, Vol. 17, No. 97, 09.2005, p. 204-214.

Research output: Contribution to journalArticle

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