Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in arabidopsis guard cells

Mohammad Mahbub Islam, Chiharu Tani, Megumi Watanabe-Sugimoto, Misugi Uraji, Md Sarwar Jahan, Choji Masuda, Yoshimasa Nakamura, Izumi Mori, Yoshiyuki Murata

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Thioglucoside glucohydrolase (myrosinase), TGG1, is a strikingly abundant protein in Arabidopsis guard cells. We investigated responses of tgg1-3, tgg2-1 and tgg1-3 tgg2-1 mutants to abscisic acid (ABA) and methyl jasmonate (MeJA) to clarify whether two myrosinases, TGG1 and TGG2, function during stomatal closure. ABA, MeJA and H2O2 induced stomatal closure in wild type, tgg1-3 and tgg2-1, but failed to induce stomatal closure in tgg1-3 tgg2-1. All mutants and wild type showed Ca2-induced stomatal closure and ABA-induced reactive oxygen species (ROS)production. A model is discussed in which two myrosinases redundantly function downstream of ROS production and upstream of cytosolic Ca2 elevation in ABA and MeJA signaling in guard cells.

Original languageEnglish
Pages (from-to)1171-1175
Number of pages5
JournalPlant and Cell Physiology
Volume50
Issue number6
DOIs
Publication statusPublished - Jun 2009

Fingerprint

Abscisic Acid
methyl jasmonate
guard cells
Arabidopsis
abscisic acid
thioglucosidase
reactive oxygen species
Reactive Oxygen Species
Thioglucosides
Arabidopsis Proteins
calcium
mutants
proteins

Keywords

  • Abscisic acid
  • Arabidopsis thaliana
  • Guard cells
  • Methyl jasmonate
  • Myrosinase
  • Reactive oxygen species

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in arabidopsis guard cells. / Islam, Mohammad Mahbub; Tani, Chiharu; Watanabe-Sugimoto, Megumi; Uraji, Misugi; Jahan, Md Sarwar; Masuda, Choji; Nakamura, Yoshimasa; Mori, Izumi; Murata, Yoshiyuki.

In: Plant and Cell Physiology, Vol. 50, No. 6, 06.2009, p. 1171-1175.

Research output: Contribution to journalArticle

Islam, Mohammad Mahbub ; Tani, Chiharu ; Watanabe-Sugimoto, Megumi ; Uraji, Misugi ; Jahan, Md Sarwar ; Masuda, Choji ; Nakamura, Yoshimasa ; Mori, Izumi ; Murata, Yoshiyuki. / Myrosinases, TGG1 and TGG2, redundantly function in ABA and MeJA signaling in arabidopsis guard cells. In: Plant and Cell Physiology. 2009 ; Vol. 50, No. 6. pp. 1171-1175.
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