Myocardial microvascular perfusion after transfusion of liposome-encapsulated hemoglobin evaluated in cross-circulated rat hearts using tracer digital radiography

Takahisa Asano, Takeshi Matsumoto, Hiroyuki Tachibana, Mami Takemoto, Fumihiko Kajiya

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The effect of hemodilution with Neo Red Cell (NRC, liposome-encapsulated hemoglobin) on myocardial perfusion was evaluated in cross-circulated rat hearts under 300-bpm pacing and 100-mmHg perfusion pressure. In NRC-transfused hearts (n = 5), NRC volume fraction and hematocrit were 9% ± 3% and 22% ± 4%, respectively; the latter decreased from 43% ± 3% before NRC transfusion. Coronary perfusion rate and left ventricular isovolemic developed pressure increased after NRC transfusion to 4.6 ± 1.0 ml/min/g and 127 ± 32 mmHg from basal values of 2.5 ± 0.3 ml/min/g and 115 ± 28 mmHg, respectively. In contrast, the flow increase during reperfusion following 30-s flow cessation decreased from 74% ± 24% to 64% ± 24%. The arteriovenous difference in O2 saturation was slightly higher after NRC transfusion. Within-layer regional flow distributions from subepicardium to subendocardium assessed by tracer digital radiography (100-μm resolution) showed that coefficients of variation of flows in 400 X 400-μm regions were 0.41 ± 0.10 in NRC-transfused hearts and 0.54 ± 0.11 in nontransfused hearts (n = 5); i.e., the myocardial flow distribution was more uniform in NRC-transfused hearts. These results suggest that NRC is superior to erythrocytes in terms of the homogenization of O 2 delivery, indicating its potential therapeutic value in myocardial microcirculatory failure.

Original languageEnglish
Pages (from-to)145-148
Number of pages4
JournalJournal of Artificial Organs
Volume7
Issue number3
DOIs
Publication statusPublished - Sep 2004
Externally publishedYes

Fingerprint

Radiographic Image Enhancement
Liposomes
Radiography
Hemoglobin
Rats
Hemoglobins
Perfusion
Pressure
Hemodilution
Hematocrit
Reperfusion
Volume fraction
Heart Failure
Erythrocytes
Cells

Keywords

  • Cross-circulation model
  • Liposome-encapsulated hemoglobin
  • Myocardial flow heterogeneity
  • Tracer digital radiography

ASJC Scopus subject areas

  • Biophysics

Cite this

Myocardial microvascular perfusion after transfusion of liposome-encapsulated hemoglobin evaluated in cross-circulated rat hearts using tracer digital radiography. / Asano, Takahisa; Matsumoto, Takeshi; Tachibana, Hiroyuki; Takemoto, Mami; Kajiya, Fumihiko.

In: Journal of Artificial Organs, Vol. 7, No. 3, 09.2004, p. 145-148.

Research output: Contribution to journalArticle

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abstract = "The effect of hemodilution with Neo Red Cell (NRC, liposome-encapsulated hemoglobin) on myocardial perfusion was evaluated in cross-circulated rat hearts under 300-bpm pacing and 100-mmHg perfusion pressure. In NRC-transfused hearts (n = 5), NRC volume fraction and hematocrit were 9{\%} ± 3{\%} and 22{\%} ± 4{\%}, respectively; the latter decreased from 43{\%} ± 3{\%} before NRC transfusion. Coronary perfusion rate and left ventricular isovolemic developed pressure increased after NRC transfusion to 4.6 ± 1.0 ml/min/g and 127 ± 32 mmHg from basal values of 2.5 ± 0.3 ml/min/g and 115 ± 28 mmHg, respectively. In contrast, the flow increase during reperfusion following 30-s flow cessation decreased from 74{\%} ± 24{\%} to 64{\%} ± 24{\%}. The arteriovenous difference in O2 saturation was slightly higher after NRC transfusion. Within-layer regional flow distributions from subepicardium to subendocardium assessed by tracer digital radiography (100-μm resolution) showed that coefficients of variation of flows in 400 X 400-μm regions were 0.41 ± 0.10 in NRC-transfused hearts and 0.54 ± 0.11 in nontransfused hearts (n = 5); i.e., the myocardial flow distribution was more uniform in NRC-transfused hearts. These results suggest that NRC is superior to erythrocytes in terms of the homogenization of O 2 delivery, indicating its potential therapeutic value in myocardial microcirculatory failure.",
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N2 - The effect of hemodilution with Neo Red Cell (NRC, liposome-encapsulated hemoglobin) on myocardial perfusion was evaluated in cross-circulated rat hearts under 300-bpm pacing and 100-mmHg perfusion pressure. In NRC-transfused hearts (n = 5), NRC volume fraction and hematocrit were 9% ± 3% and 22% ± 4%, respectively; the latter decreased from 43% ± 3% before NRC transfusion. Coronary perfusion rate and left ventricular isovolemic developed pressure increased after NRC transfusion to 4.6 ± 1.0 ml/min/g and 127 ± 32 mmHg from basal values of 2.5 ± 0.3 ml/min/g and 115 ± 28 mmHg, respectively. In contrast, the flow increase during reperfusion following 30-s flow cessation decreased from 74% ± 24% to 64% ± 24%. The arteriovenous difference in O2 saturation was slightly higher after NRC transfusion. Within-layer regional flow distributions from subepicardium to subendocardium assessed by tracer digital radiography (100-μm resolution) showed that coefficients of variation of flows in 400 X 400-μm regions were 0.41 ± 0.10 in NRC-transfused hearts and 0.54 ± 0.11 in nontransfused hearts (n = 5); i.e., the myocardial flow distribution was more uniform in NRC-transfused hearts. These results suggest that NRC is superior to erythrocytes in terms of the homogenization of O 2 delivery, indicating its potential therapeutic value in myocardial microcirculatory failure.

AB - The effect of hemodilution with Neo Red Cell (NRC, liposome-encapsulated hemoglobin) on myocardial perfusion was evaluated in cross-circulated rat hearts under 300-bpm pacing and 100-mmHg perfusion pressure. In NRC-transfused hearts (n = 5), NRC volume fraction and hematocrit were 9% ± 3% and 22% ± 4%, respectively; the latter decreased from 43% ± 3% before NRC transfusion. Coronary perfusion rate and left ventricular isovolemic developed pressure increased after NRC transfusion to 4.6 ± 1.0 ml/min/g and 127 ± 32 mmHg from basal values of 2.5 ± 0.3 ml/min/g and 115 ± 28 mmHg, respectively. In contrast, the flow increase during reperfusion following 30-s flow cessation decreased from 74% ± 24% to 64% ± 24%. The arteriovenous difference in O2 saturation was slightly higher after NRC transfusion. Within-layer regional flow distributions from subepicardium to subendocardium assessed by tracer digital radiography (100-μm resolution) showed that coefficients of variation of flows in 400 X 400-μm regions were 0.41 ± 0.10 in NRC-transfused hearts and 0.54 ± 0.11 in nontransfused hearts (n = 5); i.e., the myocardial flow distribution was more uniform in NRC-transfused hearts. These results suggest that NRC is superior to erythrocytes in terms of the homogenization of O 2 delivery, indicating its potential therapeutic value in myocardial microcirculatory failure.

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