Mycoreovirus genome alterations: Similarities to and differences from rearrangements reported for other reovi ruses

Toru Tanaka, Ana Eusebio-Cope, Liying Sun, Nobuhiro Suzuki

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The family Reoviridae is one of the largest virus families with genomes composed of 9-12 double-stranded RNA segments. It includes members infecting organisms from protists to humans. It is well known that reovirus genomes are prone to various types of genome alterations including intragenic rearrangement and reassortment under laboratory and natural conditions. Recently distinct genetic alterations were reported for members of the genus Mycoreovirus, Mycoreovirus 1 (MyRV1), and MyRV3 with 11 (S1-S11) and 12 genome segments (S1-S12), respectively. While MyRV3 S8 is lost during subculturing of infected host fungal strains, MyRV1 rearrangements undergo alterations spontaneously and inducibly. The inducible MyRV1 rearrangements are different from any other previous examples of reovirus rearrangements in their dependence on an unrelated virus factor, a multifunctional protein, p29, encoded by a distinct virus Cryphonectria parasitica hypovirus 1 (CHV1). A total of 5 MyRV1 variants with genome rearranged segments (S1-S3, S6 and S10) are generated in the background of a single viral strain in the presence of CHV1 p29 supplied either trans-genically or by coinfection. MyRV1 S4 and S10 are rearranged, albeit very infrequently, in a CHV1 p29 independent fashion. A variant of MyRV1 with substantial deletions in both S4 and S10, generated through a combined reassortment and rearrangement approach, shows comparable replication levels to the wild-type MyRV1. In vivo and in vitro interactions of CHV1 p29 and MyRV1 VP9 are implicated in the induction of MyRV1 rearrangements. However, the mechanism underlying p29-mediated rearrangements remains largely unknown. MyRV1 S4 rearrangements spontaneously occurred independently of CHV1 p29. In the absence of reverse genetics systems for mycoreoviruses, molecular and biological characterization of these MyRV1 and MyRV3 variants contribute to functional analyses of the protein products encoded by those rearranged segments.

Original languageEnglish
JournalFrontiers in Microbiology
Volume3
Issue numberJUN
DOIs
Publication statusPublished - 2012

Fingerprint

Reoviridae
Genome
S 10
Viruses
S 6
Reverse Genetics
Double-Stranded RNA

Keywords

  • Chestnut blight
  • Cryphonectria parasitica
  • Dsrna
  • Hypovirus
  • Mycoreovirus
  • Papain-like protease p29
  • Rearrangement
  • Reovirus

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Mycoreovirus genome alterations : Similarities to and differences from rearrangements reported for other reovi ruses. / Tanaka, Toru; Eusebio-Cope, Ana; Sun, Liying; Suzuki, Nobuhiro.

In: Frontiers in Microbiology, Vol. 3, No. JUN, 2012.

Research output: Contribution to journalArticle

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