Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction

Ana Eusebio-Cope, Liying Sun, Bradley I. Hillman, Nobuhiro Suzuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557-2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4, albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80-90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent.

Original languageEnglish
Pages (from-to)399-408
Number of pages10
JournalVirology
Volume397
Issue number2
DOIs
Publication statusPublished - Feb 20 2010

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Keywords

  • Chestnut blight
  • Cryphonectria parasitica
  • dsRNA
  • Hypovirulence
  • Mycovirus
  • Rearrangement
  • Reovirus
  • Symptom expression
  • Virocontrol
  • Virus transmission

ASJC Scopus subject areas

  • Virology

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