Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction

Ana Eusebio-Cope, Liying Sun, Bradley I. Hillman, Nobuhiro Suzuki

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557-2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4, albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80-90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent.

Original languageEnglish
Pages (from-to)399-408
Number of pages10
JournalVirology
Volume397
Issue number2
DOIs
Publication statusPublished - Feb 20 2010

Fingerprint

Reoviridae
S 10
Proteins
Open Reading Frames
S 6
Solar System
Virus Replication
Genome
RNA
Viruses

Keywords

  • Chestnut blight
  • Cryphonectria parasitica
  • dsRNA
  • Hypovirulence
  • Mycovirus
  • Rearrangement
  • Reovirus
  • Symptom expression
  • Virocontrol
  • Virus transmission

ASJC Scopus subject areas

  • Virology

Cite this

Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction. / Eusebio-Cope, Ana; Sun, Liying; Hillman, Bradley I.; Suzuki, Nobuhiro.

In: Virology, Vol. 397, No. 2, 20.02.2010, p. 399-408.

Research output: Contribution to journalArticle

@article{86fbcc559cd341839af9437eccda0123,
title = "Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction",
abstract = "Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557-2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4, albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80-90{\%} deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75{\%} of the ORFs in two genome segments and is still replication-competent.",
keywords = "Chestnut blight, Cryphonectria parasitica, dsRNA, Hypovirulence, Mycovirus, Rearrangement, Reovirus, Symptom expression, Virocontrol, Virus transmission",
author = "Ana Eusebio-Cope and Liying Sun and Hillman, {Bradley I.} and Nobuhiro Suzuki",
year = "2010",
month = "2",
day = "20",
doi = "10.1016/j.virol.2009.11.035",
language = "English",
volume = "397",
pages = "399--408",
journal = "Virology",
issn = "0042-6822",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Mycoreovirus 1 S4-coded protein is dispensable for viral replication but necessary for efficient vertical transmission and normal symptom induction

AU - Eusebio-Cope, Ana

AU - Sun, Liying

AU - Hillman, Bradley I.

AU - Suzuki, Nobuhiro

PY - 2010/2/20

Y1 - 2010/2/20

N2 - Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557-2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4, albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80-90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent.

AB - Rearrangements of two segments, S6 and S10, of Mycoreovirus 1 (MyRV1), a member of the family Reoviridae, were previously shown to be induced at a high rate by the multifunctional protein p29 encoded by a distinct ssRNA virus, the prototype hypovirus CHV1-EP713 (Sun and Suzuki, RNA 14, 2557-2571, 2008). Here we report the occurrence of rearrangements of MyRV1 S4, albeit at a very low frequency, in the absence of CHV1 p29, resulting in internal 80-90% deletions of the open reading frame (ORF) in S4. Comparative analyses of fungal strains infected by wild-type MyRV1 and its variants carrying rearrangements of S4, S4 plus S10 and S10 indicated that S4-encoded VP4, like VP10, is non-essential for virus replication but required for efficient vertical transmission and symptom expression caused by MyRV1. This is the first example of a reovirus variant that carries deletions of over 75% of the ORFs in two genome segments and is still replication-competent.

KW - Chestnut blight

KW - Cryphonectria parasitica

KW - dsRNA

KW - Hypovirulence

KW - Mycovirus

KW - Rearrangement

KW - Reovirus

KW - Symptom expression

KW - Virocontrol

KW - Virus transmission

UR - http://www.scopus.com/inward/record.url?scp=75749131916&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=75749131916&partnerID=8YFLogxK

U2 - 10.1016/j.virol.2009.11.035

DO - 10.1016/j.virol.2009.11.035

M3 - Article

C2 - 20006368

AN - SCOPUS:75749131916

VL - 397

SP - 399

EP - 408

JO - Virology

JF - Virology

SN - 0042-6822

IS - 2

ER -