Mutations in epidermis-specific HD-ZIP IV genes affect floral organ identity in Arabidopsis thaliana

Naoko Kamata, Hitomi Okada, Yoshibumi Komeda, Taku Takahashi

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Development of the epidermis involves members of the class-IV homeodomain-leucine zipper (HD-ZIP IV) transcription factors. The Arabidopsis HD-ZIP IV family consists of 16 members, among which PROTODERMAL FACTOR 2 (PDF2) and ARABIDOPSIS THALIANA MERISTEM LAYER 1 (ATML1) play an indispensable role in the differentiation of shoot epidermal cells; however, the functions of other HD-ZIP IV genes that are also expressed specifically in the shoot epidermis remain to be fully elucidated. We constructed double mutant combinations of these HD-ZIP IV mutant alleles and found that the double mutants of pdf2-1 with homeodomain glabrous1-1 (hdg1-1), hdg2-3, hdg5-1 and hdg12-2 produced abnormal flowers with sepaloid petals and carpelloid stamens in association with the reduced expression of the petal and stamen identity gene APETALA 3 (AP3). Expression of another petal and stamen identity gene PISTILATA (PI) was less affected in these mutants. We confirmed that AP3 expression in pdf2-1 hdg2-3 was normally induced at the initial stages of flower development, but was attenuated both in the epidermis and internal cell layers of developing flowers. As the expression of PDF2 and these HD-ZIP IV genes during floral organ formation is exclusively limited to the epidermal cell layer, these double mutations may have non-cell-autonomous effects on AP3 expression in the internal cell layers. Our results suggest that cooperative functions of PDF2 and other members of the HD-ZIP IV family in the epidermis are crucial for normal development of floral organs in Arabidopsis.

Original languageEnglish
Pages (from-to)430-440
Number of pages11
JournalPlant Journal
Volume75
Issue number3
DOIs
Publication statusPublished - Aug 2013

Keywords

  • AP3
  • Arabidopsis thaliana
  • HD-ZIP IV
  • PDF2
  • epidermis
  • floral organ identity

ASJC Scopus subject areas

  • Genetics
  • Plant Science
  • Cell Biology

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