Mutationl analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase

Mayumi Matsubara, Tamon Tanaka, Hiroaki Terato, Eiji Ohmae, Shunsuke Izumi, Katsuo Katayanagi, Hiroshi Ide

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Single-strand selective monofunctional uracil-DNA glycosylase (SMUG1), previously thought to be a backup enzyme for uracil-DNA glycosylase, has recently been shown to excise 5-hydroxyuracil (hoU), 5-hydroxymethyluracil (hmU) and 5-formyluracil (fU) bearing an oxidized group at ring C5 as well as an uracil. In the present study, we used site-directed mutagenesis to construct a series of mutants of human SMUG1 (hSMUG1), and tested their activity for uracil, hoU, hmU, fU and other bases to elucidate the catalytic and damage-recognition mechanism of hSMUG1. The functional analysis of the mutants, together with the homology modeling of the hSMUG1 structure based on that determined recently for Xenopus laevis SMUG1, revealed the crucial residues for the rupture of the N-glycosidic bond (Asn85 and His239), discrimination of pyrimidine rings through π-π stacking to the base (Phe98) and specific hydrogen bonds to the Watson-Crick face of the base (Asn163) and exquisite recognition of the C5 substituent through water-bridged (uracil) or direct (hoU, Integration and fU) hydrogen bonds (Gly87-Met91). Integration of the present results and the structural data elucidates how hSMUG1 accepts uracil, hoU, hmU and fU as substrates, but not other oxidized pyrimidines such as 5-hydroxycytosine, 5-formylcytosine and thymine glycol, and intact pyrimidines such as thymine and cytosine.

Original languageEnglish
Pages (from-to)5291-5302
Number of pages12
JournalNucleic Acids Research
Volume32
Issue number17
DOIs
Publication statusPublished - Nov 12 2004
Externally publishedYes

Fingerprint

DNA Glycosylases
Uracil
Uracil-DNA Glycosidase
Pyrimidines
Hydrogen
Thymine
Cytosine
Xenopus laevis
Site-Directed Mutagenesis
Rupture
5-formyluracil
5-hydroxyuracil
Water
Enzymes
5-hydroxymethyluracil

ASJC Scopus subject areas

  • Genetics

Cite this

Mutationl analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase. / Matsubara, Mayumi; Tanaka, Tamon; Terato, Hiroaki; Ohmae, Eiji; Izumi, Shunsuke; Katayanagi, Katsuo; Ide, Hiroshi.

In: Nucleic Acids Research, Vol. 32, No. 17, 12.11.2004, p. 5291-5302.

Research output: Contribution to journalArticle

Matsubara, M, Tanaka, T, Terato, H, Ohmae, E, Izumi, S, Katayanagi, K & Ide, H 2004, 'Mutationl analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase', Nucleic Acids Research, vol. 32, no. 17, pp. 5291-5302. https://doi.org/10.1093/nar/gkh859
Matsubara, Mayumi ; Tanaka, Tamon ; Terato, Hiroaki ; Ohmae, Eiji ; Izumi, Shunsuke ; Katayanagi, Katsuo ; Ide, Hiroshi. / Mutationl analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase. In: Nucleic Acids Research. 2004 ; Vol. 32, No. 17. pp. 5291-5302.
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