Mutants defective in chromosome partitioning in E. coli

S. Hiraga, H. Niki, R. Imamura, T. Ogura, K. Yamanaka, J. Feng, B. Ezaki, A. Jaffé

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Recent experimental results suggest that replicated daughter chromosomes (nucleoids) in Escherichia coli move non-progressively and abrupty at an early stage of the D (division) period from midcell toward the cell quarter positions, which will become the centres of the daughter cells. The chromosome positioning at the quarter positions was found to be controlled by the muk gene products. In muk mutants, normal size anucleate cells are spontaneously produced during cell division. The mukA gene is identical to the tolC gene encoding an outer membrane protein. The mukB gene codes for a 177-kDa protein. The amino acid sequence of the MukB protein deduced for the nucleotide sequence suggests that the MukB protein has five characteristic secondary structure domains: an amino-terminal globular domain containing a consensus sequence binding with ATP or another nucleotide. The central region of the protein consists of two α-helical coiled-coil domains and one globular domain. A carboxyl-terminal globular domain is rich in cysteine and positively charged residues arginine and lysine. Two MukB protein molecules might form a homodimer in the coiled-coil regions. The predicted secondary structure of the MukB protein suggests that the protein provides the force required for the positioning of nucleoides from midcell toward the cell quarters. The mukC and mukD genes are located at 88 and 41 min of the chromosome map, respectively.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalResearch in Microbiology
Volume142
Issue number2-3
DOIs
Publication statusPublished - 1991
Externally publishedYes

Fingerprint

Chromosomes
Escherichia coli
Proteins
Genes
Chromosome Positioning
Secondary Protein Structure
Consensus Sequence
Cell Size
Cell Division
Lysine
Cysteine
Arginine
Amino Acid Sequence
Membrane Proteins
Nucleotides
Adenosine Triphosphate

Keywords

  • Chromosome, Partition, muk gene
  • E. coli

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Hiraga, S., Niki, H., Imamura, R., Ogura, T., Yamanaka, K., Feng, J., ... Jaffé, A. (1991). Mutants defective in chromosome partitioning in E. coli. Research in Microbiology, 142(2-3), 189-194. https://doi.org/10.1016/0923-2508(91)90029-A

Mutants defective in chromosome partitioning in E. coli. / Hiraga, S.; Niki, H.; Imamura, R.; Ogura, T.; Yamanaka, K.; Feng, J.; Ezaki, B.; Jaffé, A.

In: Research in Microbiology, Vol. 142, No. 2-3, 1991, p. 189-194.

Research output: Contribution to journalArticle

Hiraga, S, Niki, H, Imamura, R, Ogura, T, Yamanaka, K, Feng, J, Ezaki, B & Jaffé, A 1991, 'Mutants defective in chromosome partitioning in E. coli', Research in Microbiology, vol. 142, no. 2-3, pp. 189-194. https://doi.org/10.1016/0923-2508(91)90029-A
Hiraga S, Niki H, Imamura R, Ogura T, Yamanaka K, Feng J et al. Mutants defective in chromosome partitioning in E. coli. Research in Microbiology. 1991;142(2-3):189-194. https://doi.org/10.1016/0923-2508(91)90029-A
Hiraga, S. ; Niki, H. ; Imamura, R. ; Ogura, T. ; Yamanaka, K. ; Feng, J. ; Ezaki, B. ; Jaffé, A. / Mutants defective in chromosome partitioning in E. coli. In: Research in Microbiology. 1991 ; Vol. 142, No. 2-3. pp. 189-194.
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