Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice

Hiroki Takahashi, Keiko Sasaki, Miyuki Takahashi, Naoko Shigemori, Shyunichi Honda, Hideyuki Arimitsu, Sadayuki Ochi, Naoya Ohara, Takao Tsuji

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

On single nasal immunization of mice with killed-bacillus calmette-guerin (BCG) plus a mutant Escherichia coli enterotoxin, delayed-type hypersensitivity was induced and BCG-infection decreased. Spleen cells, particularly CD4 + T cells among them produced IL-2, IFNγ and TNFα in response to the killed-BCG or purified protein derivatives. CD8+ T cells including cytotoxic T lymphocytes produced IFNγ and TNFα. However, both types of T cells reacted a little to Ag85B. The mutant induces cellular immunity to nasal killed-BCG vaccine and decreases BCG-infection. CD4+ and CD8+ T cells produce cytokines effective for tuberculosis. Although killed-BCG loses some antigens like Ag85B, nasal killed-BCG plus the mutant is useful for tuberculosis.

Original languageEnglish
Pages (from-to)3591-3598
Number of pages8
JournalVaccine
Volume24
Issue number17
DOIs
Publication statusPublished - Apr 24 2006
Externally publishedYes

Keywords

  • Heat-labile enterotoxin
  • Killed-BCG
  • Mucosal adjuvant
  • Mutant

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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