Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice

Hiroki Takahashi, Keiko Sasaki, Miyuki Takahashi, Naoko Shigemori, Shyunichi Honda, Hideyuki Arimitsu, Sadayuki Ochi, Naoya Oohara, Takao Tsuji

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

On single nasal immunization of mice with killed-bacillus calmette-guerin (BCG) plus a mutant Escherichia coli enterotoxin, delayed-type hypersensitivity was induced and BCG-infection decreased. Spleen cells, particularly CD4 + T cells among them produced IL-2, IFNγ and TNFα in response to the killed-BCG or purified protein derivatives. CD8+ T cells including cytotoxic T lymphocytes produced IFNγ and TNFα. However, both types of T cells reacted a little to Ag85B. The mutant induces cellular immunity to nasal killed-BCG vaccine and decreases BCG-infection. CD4+ and CD8+ T cells produce cytokines effective for tuberculosis. Although killed-BCG loses some antigens like Ag85B, nasal killed-BCG plus the mutant is useful for tuberculosis.

Original languageEnglish
Pages (from-to)3591-3598
Number of pages8
JournalVaccine
Volume24
Issue number17
DOIs
Publication statusPublished - Apr 24 2006
Externally publishedYes

Fingerprint

Mycobacterium bovis BCG
Enterotoxins
enterotoxins
Mycobacterium bovis
Nose
adjuvants
T-lymphocytes
Escherichia coli
T-Lymphocytes
mutants
mice
tuberculosis
Tuberculosis
BCG Vaccine
Delayed Hypersensitivity
Cytotoxic T-Lymphocytes
cytotoxic T-lymphocytes
Infection
delayed hypersensitivity
Cellular Immunity

Keywords

  • Heat-labile enterotoxin
  • Killed-BCG
  • Mucosal adjuvant
  • Mutant

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Virology
  • Infectious Diseases
  • Public Health, Environmental and Occupational Health
  • veterinary(all)

Cite this

Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice. / Takahashi, Hiroki; Sasaki, Keiko; Takahashi, Miyuki; Shigemori, Naoko; Honda, Shyunichi; Arimitsu, Hideyuki; Ochi, Sadayuki; Oohara, Naoya; Tsuji, Takao.

In: Vaccine, Vol. 24, No. 17, 24.04.2006, p. 3591-3598.

Research output: Contribution to journalArticle

Takahashi, Hiroki ; Sasaki, Keiko ; Takahashi, Miyuki ; Shigemori, Naoko ; Honda, Shyunichi ; Arimitsu, Hideyuki ; Ochi, Sadayuki ; Oohara, Naoya ; Tsuji, Takao. / Mutant Escherichia coli enterotoxin as a mucosal adjuvant induces specific Th1 responses of CD4+ and CD8+ T cells to nasal killed-bacillus calmette-guerin in mice. In: Vaccine. 2006 ; Vol. 24, No. 17. pp. 3591-3598.
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AU - Takahashi, Miyuki

AU - Shigemori, Naoko

AU - Honda, Shyunichi

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