Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle

Eisuke Ochi, Koichi Nakazato, Naokata Ishii

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Ochi, E, Nakazato, K, and Ishii, N. Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle. J Strength Cond Res 25(8): 2283-2292, 2011-We investigated the time course effects of eccentric training on muscular size, strength, and growth factor/cytokine production by using an isokinetic-exercise system for rats. Male Wistar rats (n = 34) were randomly assigned into 4 groups: 5 session eccentric-training group (ECC5S, n = 10); 5 session sham-operated group (CON5S, n = 10); 10 session eccentric-training group (ECC10S, n = 7); 10 session sham-operated group (CON10S, n = 7). In each group, a session of either training or sham operation was performed every 2 days. The training consisted of 4 sets of forced dorsiflexion (5 repetitions) combined with electric stimulation of plantar flexors. The wet weight of medial gastrocnemius muscle did not increase significantly after 5 sessions of training, whereas that after 10 sessions of training significantly increased with a concomitant increase in the cross-sectional area (CSA) of muscle fibers (weight, p <0.05; fiber CSA, p <0.001). Interleukin (IL)-6 in ECC5S and ECC10S groups showed significant increases (p <0.01), whereas those of tumor necrosis factor (TNF)-α and IL-10 did not. The phospho-stat-3 showed a significant increase in ECC10S (p <0.001) but not in ECC5S. Myostatin and follistatin also showed significant differences only between ECC10S and CON10S (p <0.05). The results showed that repeated sessions of eccentric training for 20 days cause increases in muscular size and strength associated with increases in IL-6, follistatin, phospho-stat-3, and a decrease in myostatin. The delayed responses of IL-6, myostatin, phospho-stat-3, and follistatin would be due to the chronic effects of repeated training and possibly important for muscular hypertrophy.

Original languageEnglish
Pages (from-to)2283-2292
Number of pages10
JournalJournal of Strength and Conditioning Research
Volume25
Issue number8
DOIs
Publication statusPublished - Aug 2011
Externally publishedYes

Fingerprint

Myostatin
Follistatin
Hypertrophy
Interleukin-6
Skeletal Muscle
Cytokines
Weights and Measures
Interleukin-10
Electric Stimulation
Wistar Rats
Intercellular Signaling Peptides and Proteins
Tumor Necrosis Factor-alpha
Muscles

Keywords

  • Cytokine
  • Eccentric contraction
  • Exercise
  • Growth factor
  • Muscle strength

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle. / Ochi, Eisuke; Nakazato, Koichi; Ishii, Naokata.

In: Journal of Strength and Conditioning Research, Vol. 25, No. 8, 08.2011, p. 2283-2292.

Research output: Contribution to journalArticle

@article{13a2417fbf5545f5977f3683ab608d2f,
title = "Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle",
abstract = "Ochi, E, Nakazato, K, and Ishii, N. Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle. J Strength Cond Res 25(8): 2283-2292, 2011-We investigated the time course effects of eccentric training on muscular size, strength, and growth factor/cytokine production by using an isokinetic-exercise system for rats. Male Wistar rats (n = 34) were randomly assigned into 4 groups: 5 session eccentric-training group (ECC5S, n = 10); 5 session sham-operated group (CON5S, n = 10); 10 session eccentric-training group (ECC10S, n = 7); 10 session sham-operated group (CON10S, n = 7). In each group, a session of either training or sham operation was performed every 2 days. The training consisted of 4 sets of forced dorsiflexion (5 repetitions) combined with electric stimulation of plantar flexors. The wet weight of medial gastrocnemius muscle did not increase significantly after 5 sessions of training, whereas that after 10 sessions of training significantly increased with a concomitant increase in the cross-sectional area (CSA) of muscle fibers (weight, p <0.05; fiber CSA, p <0.001). Interleukin (IL)-6 in ECC5S and ECC10S groups showed significant increases (p <0.01), whereas those of tumor necrosis factor (TNF)-α and IL-10 did not. The phospho-stat-3 showed a significant increase in ECC10S (p <0.001) but not in ECC5S. Myostatin and follistatin also showed significant differences only between ECC10S and CON10S (p <0.05). The results showed that repeated sessions of eccentric training for 20 days cause increases in muscular size and strength associated with increases in IL-6, follistatin, phospho-stat-3, and a decrease in myostatin. The delayed responses of IL-6, myostatin, phospho-stat-3, and follistatin would be due to the chronic effects of repeated training and possibly important for muscular hypertrophy.",
keywords = "Cytokine, Eccentric contraction, Exercise, Growth factor, Muscle strength",
author = "Eisuke Ochi and Koichi Nakazato and Naokata Ishii",
year = "2011",
month = "8",
doi = "10.1519/JSC.0b013e3181f1592e",
language = "English",
volume = "25",
pages = "2283--2292",
journal = "Journal of Strength and Conditioning Research",
issn = "1064-8011",
publisher = "NSCA National Strength and Conditioning Association",
number = "8",

}

TY - JOUR

T1 - Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle

AU - Ochi, Eisuke

AU - Nakazato, Koichi

AU - Ishii, Naokata

PY - 2011/8

Y1 - 2011/8

N2 - Ochi, E, Nakazato, K, and Ishii, N. Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle. J Strength Cond Res 25(8): 2283-2292, 2011-We investigated the time course effects of eccentric training on muscular size, strength, and growth factor/cytokine production by using an isokinetic-exercise system for rats. Male Wistar rats (n = 34) were randomly assigned into 4 groups: 5 session eccentric-training group (ECC5S, n = 10); 5 session sham-operated group (CON5S, n = 10); 10 session eccentric-training group (ECC10S, n = 7); 10 session sham-operated group (CON10S, n = 7). In each group, a session of either training or sham operation was performed every 2 days. The training consisted of 4 sets of forced dorsiflexion (5 repetitions) combined with electric stimulation of plantar flexors. The wet weight of medial gastrocnemius muscle did not increase significantly after 5 sessions of training, whereas that after 10 sessions of training significantly increased with a concomitant increase in the cross-sectional area (CSA) of muscle fibers (weight, p <0.05; fiber CSA, p <0.001). Interleukin (IL)-6 in ECC5S and ECC10S groups showed significant increases (p <0.01), whereas those of tumor necrosis factor (TNF)-α and IL-10 did not. The phospho-stat-3 showed a significant increase in ECC10S (p <0.001) but not in ECC5S. Myostatin and follistatin also showed significant differences only between ECC10S and CON10S (p <0.05). The results showed that repeated sessions of eccentric training for 20 days cause increases in muscular size and strength associated with increases in IL-6, follistatin, phospho-stat-3, and a decrease in myostatin. The delayed responses of IL-6, myostatin, phospho-stat-3, and follistatin would be due to the chronic effects of repeated training and possibly important for muscular hypertrophy.

AB - Ochi, E, Nakazato, K, and Ishii, N. Muscular hypertrophy and changes in cytokine production after eccentric training in the rat skeletal muscle. J Strength Cond Res 25(8): 2283-2292, 2011-We investigated the time course effects of eccentric training on muscular size, strength, and growth factor/cytokine production by using an isokinetic-exercise system for rats. Male Wistar rats (n = 34) were randomly assigned into 4 groups: 5 session eccentric-training group (ECC5S, n = 10); 5 session sham-operated group (CON5S, n = 10); 10 session eccentric-training group (ECC10S, n = 7); 10 session sham-operated group (CON10S, n = 7). In each group, a session of either training or sham operation was performed every 2 days. The training consisted of 4 sets of forced dorsiflexion (5 repetitions) combined with electric stimulation of plantar flexors. The wet weight of medial gastrocnemius muscle did not increase significantly after 5 sessions of training, whereas that after 10 sessions of training significantly increased with a concomitant increase in the cross-sectional area (CSA) of muscle fibers (weight, p <0.05; fiber CSA, p <0.001). Interleukin (IL)-6 in ECC5S and ECC10S groups showed significant increases (p <0.01), whereas those of tumor necrosis factor (TNF)-α and IL-10 did not. The phospho-stat-3 showed a significant increase in ECC10S (p <0.001) but not in ECC5S. Myostatin and follistatin also showed significant differences only between ECC10S and CON10S (p <0.05). The results showed that repeated sessions of eccentric training for 20 days cause increases in muscular size and strength associated with increases in IL-6, follistatin, phospho-stat-3, and a decrease in myostatin. The delayed responses of IL-6, myostatin, phospho-stat-3, and follistatin would be due to the chronic effects of repeated training and possibly important for muscular hypertrophy.

KW - Cytokine

KW - Eccentric contraction

KW - Exercise

KW - Growth factor

KW - Muscle strength

UR - http://www.scopus.com/inward/record.url?scp=80052739621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052739621&partnerID=8YFLogxK

U2 - 10.1519/JSC.0b013e3181f1592e

DO - 10.1519/JSC.0b013e3181f1592e

M3 - Article

VL - 25

SP - 2283

EP - 2292

JO - Journal of Strength and Conditioning Research

JF - Journal of Strength and Conditioning Research

SN - 1064-8011

IS - 8

ER -