Muscarinic receptor immunoreactivity in the superior salivatory nucleus neurons innervating the salivary glands of the rat

Hirotaka Ueda, Yoshihiro Mitoh, Masako Fujita, Motoi Kobashi, Takashi Yamashiro, Tomosada Sugimoto, Hiroyuki Ichikawa, Ryuji Matsuo

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The superior salivatory nucleus (SSN) contains preganglionic parasympathetic neurons to the submandibular and sublingual salivary glands. Cevimeline, a muscarinic acetylcholine receptor agonist, stimulates the salivary glands and is presently used as sialogogue in the treatment of dry mouth. Since cevimeline passes through the blood-brain barrier, it is also able to act on muscarinic acetylcholine receptors in the central nervous system. Our preliminary experiment using the whole-cell patch-clamp technique has shown that cevimeline excites SSN neurons in rat brain slices, suggesting that SSN neurons have muscarinic acetylcholine receptors; however, it is unclear which subtypes of muscarinic acetylcholine receptors exist in SSN neurons. In the present study, we investigated immunohistochemically muscarinic acetylcholine receptor subtypes, M1 receptor (M1R), M2R, M3R, M4R, and M5R in SSN neurons. SSN neurons innervating the salivary glands, retrogradely labeled with a fluorescent tracer from the chorda-lingual nerve, mostly expressed M3R immunoreactivity (-ir) (92.3%) but not M1R-ir. About half of such SSN neurons also showed M2R- (40.1%), M4R- (54.0%) and M5R-ir (46.0%); therefore, it is probable that SSN neurons co-express M3R-ir with at least two of the other muscarinic receptor subtypes. This is the first report to show that SSN neurons contain muscarinic acetylcholine receptors.

Original languageEnglish
Pages (from-to)42-46
Number of pages5
JournalNeuroscience Letters
Volume499
Issue number1
DOIs
Publication statusPublished - Jul 15 2011

Fingerprint

Muscarinic Receptors
Salivary Glands
Neurons
Lingual Nerve
Sublingual Gland
Cholinergic Agonists
Patch-Clamp Techniques
Blood-Brain Barrier
Mouth
Central Nervous System
Brain

Keywords

  • Immunohistochemistry
  • Muscarinic acetylcholine receptor
  • Parasympathetic preganglionic neurons
  • Submandibular glands
  • Superior salivatory nucleus

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Muscarinic receptor immunoreactivity in the superior salivatory nucleus neurons innervating the salivary glands of the rat. / Ueda, Hirotaka; Mitoh, Yoshihiro; Fujita, Masako; Kobashi, Motoi; Yamashiro, Takashi; Sugimoto, Tomosada; Ichikawa, Hiroyuki; Matsuo, Ryuji.

In: Neuroscience Letters, Vol. 499, No. 1, 15.07.2011, p. 42-46.

Research output: Contribution to journalArticle

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