MURC/Cavin-4 facilitates recruitment of ERK to caveolae and concentric cardiac hypertrophy induced by α1-adrenergic receptors

Takehiro Ogata, Daisuke Naito, Naohiko Nakanishi, Yukiko K. Hayashi, Takuya Taniguchi, Kotaro Miyagawa, Tetsuro Hamaoka, Naoki Maruyama, Satoaki Matoba, Koji Ikeda, Hiroyuki Yamada, Hidemasa Oh, Tomomi Ueyama

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The actions of catecholamines on adrenergic receptors (ARs) induce sympathetic responses, and sustained activation of the sympathetic nervous system results in disrupted circulatory homeostasis. In cardiomyocytes, α1-ARs localize to flask-shaped membrane microdomains known as "caveolae." Caveolae require both caveolin and cavin proteins for their biogenesis and function. However, the functional roles and molecular interactions of caveolar components in cardiomyocytes are poorly understood. Here, we showed that muscle-restricted coiled-coil protein (MURC)/Cavin-4 regulated α1-AR-induced cardiomyocyte hypertrophy through enhancement of ERK1/2 activation in caveolae. MURC/Cavin-4 was expressed in the caveolae and T tubules of cardiomyocytes. MURC/Cavin-4 overexpression distended the caveolae, whereas MURC/Cavin-4 was not essential for their formation. MURC/Cavin-4 deficiency attenuated cardiac hypertrophy induced by α1-AR stimulation in the presence of caveolae. Interestingly, MURC/Cavin-4 bound to α1A- and α1B-ARs as well as ERK1/2 in caveolae, and spatiotemporally modulated MEK/ERK signaling in response to α1-AR stimulation. Thus, MURC/Cavin-4 facilitates ERK1/2 recruitment to caveolae and efficient α1-AR signaling mediated by caveolae in cardiomyocytes, which provides a unique insight into the molecular mechanisms underlying caveola-mediated signaling in cardiac hypertrophy.

Original languageEnglish
Pages (from-to)3811-3816
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume111
Issue number10
DOIs
Publication statusPublished - Mar 11 2014

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Caveolae
Cardiomegaly
Adrenergic Receptors
Muscles
Cardiac Myocytes
Proteins
Caviner
Membrane Microdomains
Caveolins
Sympathetic Nervous System
Mitogen-Activated Protein Kinase Kinases
Hypertrophy
Catecholamines
Homeostasis

Keywords

  • Caveola
  • Heart
  • Plasma membrane
  • Signal transduction

ASJC Scopus subject areas

  • General

Cite this

MURC/Cavin-4 facilitates recruitment of ERK to caveolae and concentric cardiac hypertrophy induced by α1-adrenergic receptors. / Ogata, Takehiro; Naito, Daisuke; Nakanishi, Naohiko; Hayashi, Yukiko K.; Taniguchi, Takuya; Miyagawa, Kotaro; Hamaoka, Tetsuro; Maruyama, Naoki; Matoba, Satoaki; Ikeda, Koji; Yamada, Hiroyuki; Oh, Hidemasa; Ueyama, Tomomi.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, No. 10, 11.03.2014, p. 3811-3816.

Research output: Contribution to journalArticle

Ogata, T, Naito, D, Nakanishi, N, Hayashi, YK, Taniguchi, T, Miyagawa, K, Hamaoka, T, Maruyama, N, Matoba, S, Ikeda, K, Yamada, H, Oh, H & Ueyama, T 2014, 'MURC/Cavin-4 facilitates recruitment of ERK to caveolae and concentric cardiac hypertrophy induced by α1-adrenergic receptors', Proceedings of the National Academy of Sciences of the United States of America, vol. 111, no. 10, pp. 3811-3816. https://doi.org/10.1073/pnas.1315359111
Ogata, Takehiro ; Naito, Daisuke ; Nakanishi, Naohiko ; Hayashi, Yukiko K. ; Taniguchi, Takuya ; Miyagawa, Kotaro ; Hamaoka, Tetsuro ; Maruyama, Naoki ; Matoba, Satoaki ; Ikeda, Koji ; Yamada, Hiroyuki ; Oh, Hidemasa ; Ueyama, Tomomi. / MURC/Cavin-4 facilitates recruitment of ERK to caveolae and concentric cardiac hypertrophy induced by α1-adrenergic receptors. In: Proceedings of the National Academy of Sciences of the United States of America. 2014 ; Vol. 111, No. 10. pp. 3811-3816.
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AU - Hayashi, Yukiko K.

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AU - Miyagawa, Kotaro

AU - Hamaoka, Tetsuro

AU - Maruyama, Naoki

AU - Matoba, Satoaki

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