Abstract
Skeletal myogenesis is a multistep process by which multinucleated mature muscle fibers are formed from undifferentiated, mononucleated myoblasts. However, the molecular mechanisms of skeletal myogenesis have not been fully elucidated. Here, we identified muscle-restricted coiled-coil (MURC) protein as a positive regulator of myogenesis. In skeletal muscle, MURC was localized to the cytoplasm with accumulation in the Z-disc of the sarcomere. In C2C12 myoblasts, MURC expression occurred coincidentally with myogenin expression and preceded sarcomeric myosin expression during differentiation into myotubes. RNA interference (RNAi)-mediated knockdown of MURC impaired differentiation in C2C12 myoblasts, which was accompanied by impaired myogenin expression and ERK activation. Overexpression of MURC in C2C12 myoblasts resulted in the promotion of differentiation with enhanced myogenin expression and ERK activation during differentiation. During injury-induced muscle regeneration, MURC expression increased, and a higher abundance of MURC was observed in immature myofibers compared with mature myofibers. In addition, ERK was activated in regenerating tissue, and ERK activation was detected in MURC-expressing immature myofibers. These findings suggest that MURC is involved in the skeletal myogenesis that results from modulation of myogenin expression and ERK activation. MURC may play pivotal roles in the molecular mechanisms of skeletal myogenic differentiation.
| Original language | English |
|---|---|
| Journal | American Journal of Physiology - Cell Physiology |
| Volume | 295 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Aug 2008 |
| Externally published | Yes |
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Keywords
- Differentiation
- Extracellular signal-regulated kinase
- Myoblast
- Myogenin
- Skeletal muscle
ASJC Scopus subject areas
- Cell Biology
- Physiology
Cite this
MURC, a muscle-restricted coiled-coil protein, is involved in the regulation of skeletal myogenesis. / Tagawa, Masashi; Ueyama, Tomomi; Ogata, Takehiro; Takehara, Naofumi; Nakajima, Norio; Isodono, Koji; Asada, Satoshi; Takahashi, Tomosaburo; Matsubara, Hiroaki; Oh, Hidemasa.
In: American Journal of Physiology - Cell Physiology, Vol. 295, No. 2, 08.2008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - MURC, a muscle-restricted coiled-coil protein, is involved in the regulation of skeletal myogenesis
AU - Tagawa, Masashi
AU - Ueyama, Tomomi
AU - Ogata, Takehiro
AU - Takehara, Naofumi
AU - Nakajima, Norio
AU - Isodono, Koji
AU - Asada, Satoshi
AU - Takahashi, Tomosaburo
AU - Matsubara, Hiroaki
AU - Oh, Hidemasa
PY - 2008/8
Y1 - 2008/8
N2 - Skeletal myogenesis is a multistep process by which multinucleated mature muscle fibers are formed from undifferentiated, mononucleated myoblasts. However, the molecular mechanisms of skeletal myogenesis have not been fully elucidated. Here, we identified muscle-restricted coiled-coil (MURC) protein as a positive regulator of myogenesis. In skeletal muscle, MURC was localized to the cytoplasm with accumulation in the Z-disc of the sarcomere. In C2C12 myoblasts, MURC expression occurred coincidentally with myogenin expression and preceded sarcomeric myosin expression during differentiation into myotubes. RNA interference (RNAi)-mediated knockdown of MURC impaired differentiation in C2C12 myoblasts, which was accompanied by impaired myogenin expression and ERK activation. Overexpression of MURC in C2C12 myoblasts resulted in the promotion of differentiation with enhanced myogenin expression and ERK activation during differentiation. During injury-induced muscle regeneration, MURC expression increased, and a higher abundance of MURC was observed in immature myofibers compared with mature myofibers. In addition, ERK was activated in regenerating tissue, and ERK activation was detected in MURC-expressing immature myofibers. These findings suggest that MURC is involved in the skeletal myogenesis that results from modulation of myogenin expression and ERK activation. MURC may play pivotal roles in the molecular mechanisms of skeletal myogenic differentiation.
AB - Skeletal myogenesis is a multistep process by which multinucleated mature muscle fibers are formed from undifferentiated, mononucleated myoblasts. However, the molecular mechanisms of skeletal myogenesis have not been fully elucidated. Here, we identified muscle-restricted coiled-coil (MURC) protein as a positive regulator of myogenesis. In skeletal muscle, MURC was localized to the cytoplasm with accumulation in the Z-disc of the sarcomere. In C2C12 myoblasts, MURC expression occurred coincidentally with myogenin expression and preceded sarcomeric myosin expression during differentiation into myotubes. RNA interference (RNAi)-mediated knockdown of MURC impaired differentiation in C2C12 myoblasts, which was accompanied by impaired myogenin expression and ERK activation. Overexpression of MURC in C2C12 myoblasts resulted in the promotion of differentiation with enhanced myogenin expression and ERK activation during differentiation. During injury-induced muscle regeneration, MURC expression increased, and a higher abundance of MURC was observed in immature myofibers compared with mature myofibers. In addition, ERK was activated in regenerating tissue, and ERK activation was detected in MURC-expressing immature myofibers. These findings suggest that MURC is involved in the skeletal myogenesis that results from modulation of myogenin expression and ERK activation. MURC may play pivotal roles in the molecular mechanisms of skeletal myogenic differentiation.
KW - Differentiation
KW - Extracellular signal-regulated kinase
KW - Myoblast
KW - Myogenin
KW - Skeletal muscle
UR - http://www.scopus.com/inward/record.url?scp=55949135301&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=55949135301&partnerID=8YFLogxK
U2 - 10.1152/ajpcell.00188.2008
DO - 10.1152/ajpcell.00188.2008
M3 - Article
C2 - 18508909
AN - SCOPUS:55949135301
VL - 295
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0002-9513
IS - 2
ER -