Multiplex PCR and multiplex RT-PCR for inclusive detection of major swine DNA and RNA viruses in pigs with multiple infections

Hirohito Ogawa, Osamu Taira, Takuya Hirai, Hiromi Takeuchi, Aki Nagao, Yoshiki Ishikawa, Kotaro Tuchiya, Tetsuo Nunoya, Susumu Ueda

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50 Citations (Scopus)

Abstract

Multiplex PCR and multiplex RT-PCR were developed to identify nine viruses in pigs with multiple infections. These viruses are: porcine circovirus type 2 (PCV2), suid herpesvirus 1, porcine parvovirus (PPV), porcine reproductive and respiratory syndrome virus (PRRSV), Japanese encephalitis virus, porcine rotavirus A (PoRV-A), porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and Getah virus. These methods were shown to be high specificity and sensitivity. In the clinical application, a total of 75 field samples were examined by our methods and previously reported methods for PCV2, PRRSV, TGEV, and PEDV. As a result, the detection rates of our multiplex PCR and multiplex RT-PCR were higher than those of the previously reported methods. Furthermore, it was confirmed that 24 PCV2 positive samples were co-infected with other viruses, 11 with PRRSV, 10 with PPV, 2 with PoRV-A, and 1 with TGEV by a combination of multiplex PCR and multiplex RT-PCR. PPV and PoRV-A were newly detected by multiplex PCR and multiplex RT-PCR. These results suggest that the combination of our multiplex PCR and multiplex RT-PCR is useful for rapid and accurate identification of nine major pathogenic viruses in pigs with multiple infections.

Original languageEnglish
Pages (from-to)210-214
Number of pages5
JournalJournal of Virological Methods
Volume160
Issue number1-2
DOIs
Publication statusPublished - Sep 1 2009

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Keywords

  • Multiplex PCR
  • Multiplex RT-PCR
  • Porcine circovirus type 2
  • Swine viruses

ASJC Scopus subject areas

  • Virology

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