Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunhergii

Masahiro Hizume, Minoru Murata

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The ribosomal RNA (rRNA) penes were mapped on somatic chromosomes of Japanese red pine (Pinus densiflora) and Japanese black pine (P. thunbergii) by in situ hybridization using biotin-labeled 18S-25S rDNA. Cytochemical detection of the hybridized probe showed many signals in the interphase nuclei and, prophase and metaphase chromosomes in both species. In the metaphase complement of P. densiflora, the signals appeared singly at the interstitial region of seven pairs of the chromosomes. In P. thunbergii, ten strong and two weak signals appeared at the interstitial region of six pairs of the chromosomes. The hybridization sites coincided with the interstitial chromomycin As-bands or secondary constricted regions.

Original languageEnglish
Pages (from-to)389-396
Number of pages8
JournalJapanese Journal of Genetics
Volume67
Issue number5
DOIs
Publication statusPublished - Jan 1 1992

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunhergii'. Together they form a unique fingerprint.

Cite this