Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunhergii

Masahiro Hizume, Minoru Murata

    Research output: Contribution to journalArticlepeer-review

    28 Citations (Scopus)

    Abstract

    The ribosomal RNA (rRNA) penes were mapped on somatic chromosomes of Japanese red pine (Pinus densiflora) and Japanese black pine (P. thunbergii) by in situ hybridization using biotin-labeled 18S-25S rDNA. Cytochemical detection of the hybridized probe showed many signals in the interphase nuclei and, prophase and metaphase chromosomes in both species. In the metaphase complement of P. densiflora, the signals appeared singly at the interstitial region of seven pairs of the chromosomes. In P. thunbergii, ten strong and two weak signals appeared at the interstitial region of six pairs of the chromosomes. The hybridization sites coincided with the interstitial chromomycin As-bands or secondary constricted regions.

    Original languageEnglish
    Pages (from-to)389-396
    Number of pages8
    JournalJapanese Journal of Genetics
    Volume67
    Issue number5
    DOIs
    Publication statusPublished - 1992

    ASJC Scopus subject areas

    • Genetics

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