Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunbergii

M. Hizume, F. Ishida, M. Murata

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27 Citations (Scopus)

Abstract

The ribosomal RNA (rRNA) genes were mapped on somatic chromosomes of Japanese red pine (Pinus densiflora) and Japanese black pine (P. thunbergii) by in situ hybridization using biotin-labeled 18S-25S rDNA. Cytochemical detection of the hybridized probe showed many signals in the interphase nuclei and, prophase and metaphase chromosomes in both species. In the metaphase complement of P. densiflora, the signals appeared singly at the interstitial region of seven pairs of the chromosomes. In P. thunbergii, ten strong and two weak signals appeared at the interstitial region of six pairs of the chromosomes. The hybridization sites coincided with the interstitial chromomycin A3-bands or secondary constricted regions.

Original languageEnglish
Pages (from-to)389-396
Number of pages8
JournalJapanese Journal of Genetics
Volume67
Issue number5
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Pinus
rRNA Genes
Chromosomes
Metaphase
Chromomycin A3
Prophase
Interphase
Biotin
Ribosomal DNA
In Situ Hybridization
Genes

ASJC Scopus subject areas

  • Genetics

Cite this

Multiple locations of the rRNA genes in chromosomes of pines, Pinus densiflora and P. thunbergii. / Hizume, M.; Ishida, F.; Murata, M.

In: Japanese Journal of Genetics, Vol. 67, No. 5, 1992, p. 389-396.

Research output: Contribution to journalArticle

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