Multiple intracellular locations of lon protease in Arabidopsis: Evidence for the localization of AtLon4 to chloroplasts

Oren Ostersetzer, Yusuke Kato, Zach Adam, Wataru Sakamoto

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Arabidopsis contains four Lon protease-like proteins (AtLon1-AtLon4), predicted to be localized in different cellular organelles, including mitochondria, peroxisomes and plastids. A notable question is whether Lon is present in chloroplasts, since it is absent from cyanobacteria and thus appears to have been lost during the evolution of photosynthetic organisms. Based on in vivo transient assays, we found that AtLon4 is dually targeted to both mitochondria and chloroplasts. Furthermore, immunoblot analysis localized AtLon4 to the thylakoids. Thus, in spite of its absence from basal photosynthetic organisms, our results suggest the presence of Lon in plant plastids.

Original languageEnglish
Pages (from-to)881-885
Number of pages5
JournalPlant and Cell Physiology
Volume48
Issue number6
DOIs
Publication statusPublished - Jun 1 2007

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Keywords

  • AAA family
  • ATP-dependent protease
  • Chloroplasts
  • Lon
  • Mitochondria
  • Peroxisomes

ASJC Scopus subject areas

  • Physiology
  • Plant Science
  • Cell Biology

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