Multiple intracellular locations of lon protease in Arabidopsis: Evidence for the localization of AtLon4 to chloroplasts

Oren Ostersetzer, Yusuke Kato, Zach Adam, Wataru Sakamoto

    Research output: Contribution to journalArticlepeer-review

    50 Citations (Scopus)

    Abstract

    Arabidopsis contains four Lon protease-like proteins (AtLon1-AtLon4), predicted to be localized in different cellular organelles, including mitochondria, peroxisomes and plastids. A notable question is whether Lon is present in chloroplasts, since it is absent from cyanobacteria and thus appears to have been lost during the evolution of photosynthetic organisms. Based on in vivo transient assays, we found that AtLon4 is dually targeted to both mitochondria and chloroplasts. Furthermore, immunoblot analysis localized AtLon4 to the thylakoids. Thus, in spite of its absence from basal photosynthetic organisms, our results suggest the presence of Lon in plant plastids.

    Original languageEnglish
    Pages (from-to)881-885
    Number of pages5
    JournalPlant and Cell Physiology
    Volume48
    Issue number6
    DOIs
    Publication statusPublished - Jun 2007

    Keywords

    • AAA family
    • ATP-dependent protease
    • Chloroplasts
    • Lon
    • Mitochondria
    • Peroxisomes

    ASJC Scopus subject areas

    • Physiology
    • Plant Science
    • Cell Biology

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