TY - JOUR
T1 - Multiple free-radical scavenging (MULTIS) capacity in cattle serum
AU - Sueishi, Yoshimi
AU - Kamogawa, Erisa
AU - Kimura, Anna
AU - Kitahara, Go
AU - Satoh, Hiroyuki
AU - Asanuma, Taketoshi
AU - Oowada, Shigeru
N1 - Publisher Copyright:
© 2017 JCBN.
Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 2017/1
Y1 - 2017/1
N2 - Multiple free-radical scavenging (MULTIS) activity in cattle and human sera was evaluated with electron spin resonance spectros- copy. Scavenging rates against six active species, namely hydroxyl radical, superoxide anion, alkoxyl radical, alkylperoxyl radical, methyl radical, and singlet oxygen were quantified. The difference in the electron spin resonance signal intensity in the presence and absence of the serum was converted into the scavenging rates. Comparative MULTIS measurements were made in sera from eight beef cattle, three fetal calves and fifteen healthy human volunteers. Further, we determined the MULTIS value of albumin, the most abundant component in serum. MULTIS values in cattle sera indicated higher scavenging activity against most free radical species tested than human sera. In particular, cattle serum scavenging activities against superoxide and methyl radical were higher than human serum by 2.6 and 3.7 fold, respectively. In cattle serum, albumin appears to play a dominant role in MULTIS activity, but in human serum that is not the case. Previous data indicated that the abundance of uric acid in bovine blood is nearly 80% less than humans; however, this difference does not explain the deviation in MULTIS profile.
AB - Multiple free-radical scavenging (MULTIS) activity in cattle and human sera was evaluated with electron spin resonance spectros- copy. Scavenging rates against six active species, namely hydroxyl radical, superoxide anion, alkoxyl radical, alkylperoxyl radical, methyl radical, and singlet oxygen were quantified. The difference in the electron spin resonance signal intensity in the presence and absence of the serum was converted into the scavenging rates. Comparative MULTIS measurements were made in sera from eight beef cattle, three fetal calves and fifteen healthy human volunteers. Further, we determined the MULTIS value of albumin, the most abundant component in serum. MULTIS values in cattle sera indicated higher scavenging activity against most free radical species tested than human sera. In particular, cattle serum scavenging activities against superoxide and methyl radical were higher than human serum by 2.6 and 3.7 fold, respectively. In cattle serum, albumin appears to play a dominant role in MULTIS activity, but in human serum that is not the case. Previous data indicated that the abundance of uric acid in bovine blood is nearly 80% less than humans; however, this difference does not explain the deviation in MULTIS profile.
KW - Antioxidants
KW - Cattle serum
KW - MULTIS
KW - Multiple radical scavenging capacity
KW - Spin trapping
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U2 - 10.3164/jcbn.15-142
DO - 10.3164/jcbn.15-142
M3 - Article
AN - SCOPUS:85007595751
VL - 60
SP - 76
EP - 80
JO - Journal of Clinical Biochemistry and Nutrition
JF - Journal of Clinical Biochemistry and Nutrition
SN - 0912-0009
IS - 1
ER -