Multiple forms of chlorophyll-protein complexes from a thermophilic cyanobacterium Synechococcus sp

Yuichiro Takahashi, Hiroyuki Koike, Sakae Katoh

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Eight chlorophyll-proteins were resolved from the thylakoid membranes, or digitonin particles, of a thermophilic cyanobacterium Synechococcus sp. by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Six chlorophyll-proteins with slower electrophoretic mobilities were shown to be P700-chlorophyll a-protein complexes (CP1), whereas faster-moving proteins (CP2) were related to photosystem 2. Extraction of CP1 complexes from the membranes with different detergent/chlorophyll ratios and reelectrophoresis of extracted CP1 complexes indicated that the chlorophyll-proteins are closely interrelated with each other; any CP1 complex could be transformed to other CP1 complexes with faster electrophoretic mobilities. This, together with the Ferguson plot and the polypeptide composition, showed that six CP1 complexes are different in terms of polypeptide composition, oligomerization, SDS-binding, or conformation of the proteins but represent, in the order of increasing electrophoretic mobility, increasing degree of modification of the native P700-chlorophyll a-protein.

Original languageEnglish
Pages (from-to)209-218
Number of pages10
JournalArchives of Biochemistry and Biophysics
Volume219
Issue number1
DOIs
Publication statusPublished - 1982
Externally publishedYes

Fingerprint

Chlorophyll Binding Proteins
Synechococcus
Cyanobacteria
Chlorophyll
Electrophoretic mobility
Proteins
Digitonin
Peptides
Protein Conformation
Thylakoids
Membranes
Oligomerization
Sodium Dodecyl Sulfate
Detergents
Polyacrylamide Gel Electrophoresis
Electrophoresis
Chemical analysis
Conformations

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Multiple forms of chlorophyll-protein complexes from a thermophilic cyanobacterium Synechococcus sp. / Takahashi, Yuichiro; Koike, Hiroyuki; Katoh, Sakae.

In: Archives of Biochemistry and Biophysics, Vol. 219, No. 1, 1982, p. 209-218.

Research output: Contribution to journalArticle

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