Multidrug-resistance transporter AbcA secretes staphylococcus aureus cytolytic toxins

Hirono Yoshikai, Hayato Kizaki, Yuki Saito, Yosuke Omae, Kazuhisa Sekimizu, Chikara Kaito

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Phenol-soluble modulins (PSMs) are Staphylococcus aureus cytolytic toxins that lyse erythrocytes and neutrophils and have important functions in the S. aureus infectious process. The molecular mechanisms of PSM secretion, however, are not well understood. Here we report that knockout of the multidrug-resistance ABC transporter AbcA, which contributes to S. aureus resistance against antibiotics and chemicals, diminished the secreted amount of PSM, leading to the accumulation of PSM in the intracellular fraction. The amount of PSM in the culture supernatants of the abcA knockout mutants was restored by introduction of the wild-type abcA gene, whereas it was not completely restored by introduction of mutant abcA genes encoding AbcA mutant proteins carrying amino acid substitutions in the adenosine triphosphate binding motifs. The abcA knockout mutant exhibited attenuated virulence in a mouse systemic infection model. These findings suggest that the multidrug resistance transporter AbcA secretes PSMs and contributes to S. aureus virulence.

Original languageEnglish
Pages (from-to)295-304
Number of pages10
JournalJournal of Infectious Diseases
Volume213
Issue number2
DOIs
Publication statusPublished - Jan 15 2016
Externally publishedYes

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Keywords

  • cytolytic toxins
  • multidrug resistance transporter
  • phenol-soluble modulins
  • Staphylococcus aureus

ASJC Scopus subject areas

  • Immunology and Allergy
  • Infectious Diseases

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