Multidrug resistance in Klebsiella pneumoniae MGH78578 and cloning of genes responsible for the resistance

Wakano Ogawa, Dai Wei Li, Ping Yu, Anowara Begum, Tohru Mizushima, Teruo Kuroda, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Klebsiella pneumoniae MGH78578, a clinical isolate, showed high level of resistance to many antimicrobial agents. We cloned genes responsible for drug resistance from chromosomal DNA of K. pneumoniae MGH78578 by shotgun method using Escherichia coli KAM32, a drug hypersensitive strain, as host. We obtained 43 hybrid plasmids that made host cells resistant to several antimicrobial agents. We classified them into 17 groups based on growth properties in the presence of each one of 9 antimicrobial agents and on restriction patterns of each hybrid plasmid. Analysis of the cloned genes must be very useful for investigation of major parts of multidrug resistance systems including multidrug efflux pumps in K. pneumoniae MGH78578 in which genome sequence is available.

Original languageEnglish
Pages (from-to)1505-1508
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Volume28
Issue number8
DOIs
Publication statusPublished - Aug 2005

Fingerprint

Klebsiella pneumoniae
Multiple Drug Resistance
Anti-Infective Agents
Organism Cloning
Plasmids
Genes
Firearms
Drug Resistance
Genome
Escherichia coli
DNA
Growth
Pharmaceutical Preparations

Keywords

  • Gene cloning
  • Klebsiella pneumoniae
  • Multidrug efflux pump
  • Multidrug resistance

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Multidrug resistance in Klebsiella pneumoniae MGH78578 and cloning of genes responsible for the resistance. / Ogawa, Wakano; Li, Dai Wei; Yu, Ping; Begum, Anowara; Mizushima, Tohru; Kuroda, Teruo; Tsuchiya, Tomofusa.

In: Biological and Pharmaceutical Bulletin, Vol. 28, No. 8, 08.2005, p. 1505-1508.

Research output: Contribution to journalArticle

Ogawa, Wakano ; Li, Dai Wei ; Yu, Ping ; Begum, Anowara ; Mizushima, Tohru ; Kuroda, Teruo ; Tsuchiya, Tomofusa. / Multidrug resistance in Klebsiella pneumoniae MGH78578 and cloning of genes responsible for the resistance. In: Biological and Pharmaceutical Bulletin. 2005 ; Vol. 28, No. 8. pp. 1505-1508.
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