Multi-faceted analyses of a highly metastatic human lung cancer cell line NCI-H460-LNM35 suggest mimicry of inflammatory cells in metastasis

Ken Ichi Kozaki; Katsumi Koshikawa; Yoshio Tatematsu; Osamu Miyaishi; Hiroko Saito; Toyoaki Hida; Hirotaka Osada; Takashi Takahashi

doi:10.1038/sj.onc.1204561

Multi-faceted analyses of a highly metastatic human lung cancer cell line NCI-H460-LNM35 suggest mimicry of inflammatory cells in metastasis

Ken Ichi Kozaki, Katsumi Koshikawa, Yoshio Tatematsu, Osamu Miyaishi, Hiroko Saito, Toyoaki Hida, Hirotaka Osada, Takashi Takahashi

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Abstract

This study established and characterized low-metastatic revertant and parental clones of a highly metastatic human lung cancer cell line, NCI-H460-LNM35 (hereafter referred to as LNM35). Expression-profiling analysis revealed that up-regulation of various proinflammatory cytokines and angiogenic chemotactic chemokines was present in LNM35. Further, while COX-2 itself is known to be inducible in inflammation, COX-2 expression levels correlated well with the capabilities of these clones for not only in vitro motility and invasion but also in vivo metastasis, and COX-2 inhibitors were shown for the first time to reduce lung cancer metastasis in vivo. These findings suggest that lung cancer cells may mimic inflammatory cells in the process of metastasis.

Original language	English
Pages (from-to)	4228-4234
Number of pages	7
Journal	Oncogene
Volume	20
Issue number	31
DOIs	https://doi.org/10.1038/sj.onc.1204561
Publication status	Published - Jul 12 2001

Keywords

Expression profiling
Human lung cancer
Inflammation
Invasion
Metastasis

ASJC Scopus subject areas

Molecular Biology
Genetics
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1038/sj.onc.1204561

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title = "Multi-faceted analyses of a highly metastatic human lung cancer cell line NCI-H460-LNM35 suggest mimicry of inflammatory cells in metastasis",

abstract = "This study established and characterized low-metastatic revertant and parental clones of a highly metastatic human lung cancer cell line, NCI-H460-LNM35 (hereafter referred to as LNM35). Expression-profiling analysis revealed that up-regulation of various proinflammatory cytokines and angiogenic chemotactic chemokines was present in LNM35. Further, while COX-2 itself is known to be inducible in inflammation, COX-2 expression levels correlated well with the capabilities of these clones for not only in vitro motility and invasion but also in vivo metastasis, and COX-2 inhibitors were shown for the first time to reduce lung cancer metastasis in vivo. These findings suggest that lung cancer cells may mimic inflammatory cells in the process of metastasis.",

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author = "Kozaki, {Ken Ichi} and Katsumi Koshikawa and Yoshio Tatematsu and Osamu Miyaishi and Hiroko Saito and Toyoaki Hida and Hirotaka Osada and Takashi Takahashi",

note = "Funding Information: We would like to thank K Matsuo for his advice on the biostatistical analyses. We are also grateful to H Konishi and Y Yatabe for their critical reading of this manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science and Culture of Japan.",

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doi = "10.1038/sj.onc.1204561",

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AU - Kozaki, Ken Ichi

AU - Koshikawa, Katsumi

AU - Tatematsu, Yoshio

AU - Miyaishi, Osamu

AU - Saito, Hiroko

AU - Hida, Toyoaki

AU - Osada, Hirotaka

AU - Takahashi, Takashi

N1 - Funding Information: We would like to thank K Matsuo for his advice on the biostatistical analyses. We are also grateful to H Konishi and Y Yatabe for their critical reading of this manuscript. This work was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science and Culture of Japan.

PY - 2001/7/12

Y1 - 2001/7/12

N2 - This study established and characterized low-metastatic revertant and parental clones of a highly metastatic human lung cancer cell line, NCI-H460-LNM35 (hereafter referred to as LNM35). Expression-profiling analysis revealed that up-regulation of various proinflammatory cytokines and angiogenic chemotactic chemokines was present in LNM35. Further, while COX-2 itself is known to be inducible in inflammation, COX-2 expression levels correlated well with the capabilities of these clones for not only in vitro motility and invasion but also in vivo metastasis, and COX-2 inhibitors were shown for the first time to reduce lung cancer metastasis in vivo. These findings suggest that lung cancer cells may mimic inflammatory cells in the process of metastasis.

AB - This study established and characterized low-metastatic revertant and parental clones of a highly metastatic human lung cancer cell line, NCI-H460-LNM35 (hereafter referred to as LNM35). Expression-profiling analysis revealed that up-regulation of various proinflammatory cytokines and angiogenic chemotactic chemokines was present in LNM35. Further, while COX-2 itself is known to be inducible in inflammation, COX-2 expression levels correlated well with the capabilities of these clones for not only in vitro motility and invasion but also in vivo metastasis, and COX-2 inhibitors were shown for the first time to reduce lung cancer metastasis in vivo. These findings suggest that lung cancer cells may mimic inflammatory cells in the process of metastasis.

KW - Expression profiling

KW - Human lung cancer

KW - Inflammation

KW - Invasion

KW - Metastasis

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Multi-faceted analyses of a highly metastatic human lung cancer cell line NCI-H460-LNM35 suggest mimicry of inflammatory cells in metastasis

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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