mTrs130 is a component of a mammalian TRAPPII complex, a Rab1 GEF that binds to COPI-coated vesicles

Akinori Yamasaki, Shekar Menon, Sidney Yu, Jemima Barrowman, Timo Meerloo, Viola Oorschot, Judith Klumperman, Ayano Satoh, Susan Ferro-Novick

Research output: Contribution to journalArticle

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Abstract

The GTPase Rab1 regulates endoplasmic reticulum-Golgi and early Golgi traffic. The guanine nucleotide exchange factor (GEF) or factors that activate Rab1 at these stages of the secretory pathway are currently unknown. Trs130p is a subunit of the yeast TRAPPII (transport protein particle II) complex, a multisubunit tethering complex that is a GEF for the Rab1 homologue Ypt1p. Here, we show that mammalian Trs130 (mTrs130) is a component of an analogous TRAPP complex in mammalian cells, and we describe for the first time the role that this complex plays in membrane traffic. mTRAPPII is enriched on COPI (Coat Protein I)-coated vesicles and buds, but not Golgi cisternae, and it specifically activates Rab1. In addition, we find that mTRAPPII binds to γ1COP, a COPI coat adaptor subunit. The depletion of mTrs130 by short hairpin RNA leads to an increase of vesicles in the vicinity of the Golgi and the accumulation of cargo in an early Golgi compartment. We propose that mTRAPPII is a Rab1 GEF that tethers COPI-coated vesicles to early Golgi membranes.

Original languageEnglish
Pages (from-to)4205-4215
Number of pages11
JournalMolecular Biology of the Cell
Volume20
Issue number19
DOIs
Publication statusPublished - Oct 1 2009

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Coated Vesicles
Guanine Nucleotide Exchange Factors
Capsid Proteins
Carrier Proteins
Fungal Proteins
Membranes
Secretory Pathway
GTP Phosphohydrolases
Endoplasmic Reticulum
Small Interfering RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Yamasaki, A., Menon, S., Yu, S., Barrowman, J., Meerloo, T., Oorschot, V., ... Ferro-Novick, S. (2009). mTrs130 is a component of a mammalian TRAPPII complex, a Rab1 GEF that binds to COPI-coated vesicles. Molecular Biology of the Cell, 20(19), 4205-4215. https://doi.org/10.1091/mbc.E09-05-0387

mTrs130 is a component of a mammalian TRAPPII complex, a Rab1 GEF that binds to COPI-coated vesicles. / Yamasaki, Akinori; Menon, Shekar; Yu, Sidney; Barrowman, Jemima; Meerloo, Timo; Oorschot, Viola; Klumperman, Judith; Satoh, Ayano; Ferro-Novick, Susan.

In: Molecular Biology of the Cell, Vol. 20, No. 19, 01.10.2009, p. 4205-4215.

Research output: Contribution to journalArticle

Yamasaki, A, Menon, S, Yu, S, Barrowman, J, Meerloo, T, Oorschot, V, Klumperman, J, Satoh, A & Ferro-Novick, S 2009, 'mTrs130 is a component of a mammalian TRAPPII complex, a Rab1 GEF that binds to COPI-coated vesicles', Molecular Biology of the Cell, vol. 20, no. 19, pp. 4205-4215. https://doi.org/10.1091/mbc.E09-05-0387
Yamasaki, Akinori ; Menon, Shekar ; Yu, Sidney ; Barrowman, Jemima ; Meerloo, Timo ; Oorschot, Viola ; Klumperman, Judith ; Satoh, Ayano ; Ferro-Novick, Susan. / mTrs130 is a component of a mammalian TRAPPII complex, a Rab1 GEF that binds to COPI-coated vesicles. In: Molecular Biology of the Cell. 2009 ; Vol. 20, No. 19. pp. 4205-4215.
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