Abstract
The cochlear stria vascularis is essential for the normal function of hair cells. mRNA encoding the ClC-K1 Cl- channel, previously thought to be found only in the kidney, was detected in epithelial marginal cells of the rat stria vascularis by single cell RT-PCR. When Cl- currents were recorded from rat marginal cells by the whole-cell patch clamp method, the steady-state currents showed weak outward rectification and an ion selectivity sequence of SCN->Br-=Cl->F->NO3->I->gluconate-. The Cl- currents were regulated by extracellular Ca2+ and pH. These characteristics resemble those reported for the currents recorded from Xenopus oocytes expressing ClC-K1 Cl- channels. These data together suggest that ClC-K1 Cl- channels may contribute to the whole-cell currents of marginal cells. Copyright (C) 2000 Elsevier Science Ireland Ltd.
Original language | English |
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Pages (from-to) | 171-174 |
Number of pages | 4 |
Journal | Neuroscience Letters |
Volume | 284 |
Issue number | 3 |
DOIs | |
Publication status | Published - Apr 28 2000 |
Externally published | Yes |
Keywords
- Endolymph
- Inner ear
- Ion transport
- Patch clamp
- Single cell RT-PCR
ASJC Scopus subject areas
- Neuroscience(all)